Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators

Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators

Enriching mitochondrial DNA (mtDNA) for sequencing entire mitochondrial genomes (mitogenomes) can be achieved by single long-range PCR. This avoids interference from the omnipresent nuclear mtDNA sequences (NUMTs). The approach is currently restricted to the use of samples collected from humans and...

Full description

Bibliographic Details
Main Authors: Sarah V. Emser, Helmut Schaschl, Eva Millesi, Ralf Steinborn
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Genetics
Subjects:
mitogenome
long-range PCR
back-to-back amplification primers
next-generation sequencing
mitochondrial-derived peptides
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.685806/full

Similar Items

Similar Items