Quantitative <sup>1</sup>H Nuclear Magnetic Resonance (qNMR) of Aromatic Amino Acids for Protein Quantification

Hydrolysis of protein samples into amino acids facilitates the use of NMR spectroscopy for protein and peptide quantification. Different conditions have been tested for quantifying aromatic amino acids and proteins. The pH-dependent signal shifts in the aromatic region of amino acid samples were exa...

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Bibliographic Details
Main Authors: Teodor Tchipilov, Klas Meyer, Michael G. Weller
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/6/1/11