INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES
Liver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage. It was shown previously th...
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| Format: | Article |
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Central Research Institute for Epidemiology
2018-08-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/202/112 |
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| author | D. S. Kostyushev S. A. Brezgin A. P. Kostyusheva A. D. Lipatnikov V. N. Simirskii N. A. Mamonova E. V. Volchkova V. V. Maleyev V. P. Chulanov |
| author_facet | D. S. Kostyushev S. A. Brezgin A. P. Kostyusheva A. D. Lipatnikov V. N. Simirskii N. A. Mamonova E. V. Volchkova V. V. Maleyev V. P. Chulanov |
| author_sort | D. S. Kostyushev |
| collection | DOAJ |
| description | Liver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage. It was shown previously that generation of phosphorylated histone H2AX foci is a reliable marker of DSBs. The aim of this study was to analyse generation of yH2AX foci in HBV and hepatitis D virus (HDV) infection in vitro and in liver biopsies of patients with CHB and CHB with delta-agent (CHD). Human hepatoma cell line HepG2-1.1merHBV with activated HBV life cycle was used to perform real-time PCR for analysis of pregenomic RNA, HBV DNA, HBV cccDNA and for immunocytochemical analysis of yH2AX. Liver biopsies from CHB and CHD patients were analyzed to confirm the results. HBV induces multiple discrete yH2AX foci in HepG2-1.1merHBV cells in vitro and in biopsies of CHB and CHB+D patients. The ratio of hepatocytes w/o yH2AX foci is significantly lower (49,9+/-12,3% vs. 85,5+/-0,9%, p |
| first_indexed | 2024-03-13T00:08:27Z |
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| id | doaj.art-290b2c9b33e84d7aba2e4e22b29ecc23 |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:27Z |
| publishDate | 2018-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-290b2c9b33e84d7aba2e4e22b29ecc232023-07-12T20:00:15ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972018-08-0163416517010.18821/0507-4088-2018-63-4-165-170182INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSESD. S. Kostyushev0S. A. Brezgin1A. P. Kostyusheva2A. D. Lipatnikov3V. N. Simirskii4N. A. Mamonova5E. V. Volchkova6V. V. Maleyev7V. P. Chulanov8Central Research Institute of EpidemiologyCentral Research Institute of EpidemiologyCentral Research Institute of EpidemiologyCentral Research Institute of EpidemiologyKoltzov Institute of Developmental BiologyCentral Research Institute of EpidemiologyI.M. Sechenov First State Medical UniversityCentral Research Institute of EpidemiologyCentral Research Institute of EpidemiologyLiver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage. It was shown previously that generation of phosphorylated histone H2AX foci is a reliable marker of DSBs. The aim of this study was to analyse generation of yH2AX foci in HBV and hepatitis D virus (HDV) infection in vitro and in liver biopsies of patients with CHB and CHB with delta-agent (CHD). Human hepatoma cell line HepG2-1.1merHBV with activated HBV life cycle was used to perform real-time PCR for analysis of pregenomic RNA, HBV DNA, HBV cccDNA and for immunocytochemical analysis of yH2AX. Liver biopsies from CHB and CHD patients were analyzed to confirm the results. HBV induces multiple discrete yH2AX foci in HepG2-1.1merHBV cells in vitro and in biopsies of CHB and CHB+D patients. The ratio of hepatocytes w/o yH2AX foci is significantly lower (49,9+/-12,3% vs. 85,5+/-0,9%, phttps://virusjour.crie.ru/jour/article/viewFile/202/112yh2axhepatitis b virushepatitis d virusdouble strand breaksyh2axgenome damagehepatocyteshepatoma cells |
| spellingShingle | D. S. Kostyushev S. A. Brezgin A. P. Kostyusheva A. D. Lipatnikov V. N. Simirskii N. A. Mamonova E. V. Volchkova V. V. Maleyev V. P. Chulanov INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES Вопросы вирусологии yh2ax hepatitis b virus hepatitis d virus double strand breaks yh2ax genome damage hepatocytes hepatoma cells |
| title | INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES |
| title_full | INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES |
| title_fullStr | INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES |
| title_full_unstemmed | INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES |
| title_short | INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES |
| title_sort | increased formation of phosphorylated h2ax foci in nuclei of cells infected by hepatitis b and b d viruses |
| topic | yh2ax hepatitis b virus hepatitis d virus double strand breaks yh2ax genome damage hepatocytes hepatoma cells |
| url | https://virusjour.crie.ru/jour/article/viewFile/202/112 |
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