Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types

The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and to measure endogenous protein dynamics in single cel...

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Bibliographic Details
Main Authors: Sascha Conic, Dominique Desplancq, László Tora, Etienne Weiss
Format: Article
Language:English
Published: Bio-protocol LLC 2018-11-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3069&type=0