Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity
PURPOSE:To create in vitro a model to generate acidosis by CO2bubbling "organ chambers", which would be useful for researchers that aim to study the effects of acid-base disturbs on the endothelium-dependent vascular reactivity.METHODS:Eighteen male Wistar rats (230-280g) were housed, befo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014-11-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001100711&tlng=en |
| _version_ | 1830512932671193088 |
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| author | Tales Rubens de Nadai Ana Paula Cassiano Silveira Ariadne Santana e Neves Monteiro Debora Ribeiro Campos Marco Tulio Rezende de Carvalho Agnes Afrodite Sumarelli Albuquerque Andrea Carla Celotto Paulo Roberto Barbosa Evora |
| author_facet | Tales Rubens de Nadai Ana Paula Cassiano Silveira Ariadne Santana e Neves Monteiro Debora Ribeiro Campos Marco Tulio Rezende de Carvalho Agnes Afrodite Sumarelli Albuquerque Andrea Carla Celotto Paulo Roberto Barbosa Evora |
| author_sort | Tales Rubens de Nadai |
| collection | DOAJ |
| description | PURPOSE:To create in vitro a model to generate acidosis by CO2bubbling "organ chambers", which would be useful for researchers that aim to study the effects of acid-base disturbs on the endothelium-dependent vascular reactivity.METHODS:Eighteen male Wistar rats (230-280g) were housed, before the experiments, under standard laboratory conditions (12h light/dark cycle at 21°C), with free access to food and water. The protocol for promoting in vitro respiratory acidosis was carried out by bubbling increased concentrations of CO2. The target was to achieve an ideal way to decrease the pH gradually to a value of approximately 6.6.It was used, initially, a gas blender varying concentrations of the carbogenic mixture (95% O2 + 5% CO2) and pure CO2.RESULTS:1) 100% CO2, pH variation very fast, pH minimum 6.0; 2) 90%CO2 pH variation bit slower, pH minimum6.31; 3) 70%CO2, pH variation slower, pH minimum 6.32; 4) 50% CO2, pH variation slower, pH minimum 6:42; 5) 40 %CO2, Adequate record, pH minimum 6.61, and; 6) 30 %CO2 could not reach values below pH minimum 7.03. Based on these data the gas mixture (O2 60% + CO2 40%) was adopted,CONCLUSION: This gas mixture (O2 60% + CO2 40%) was effective in inducing respiratory acidosis at a speed that made, possible the recording of isometric force. |
| first_indexed | 2024-12-22T02:35:31Z |
| format | Article |
| id | doaj.art-2932002ecd794f39a4aa02cadec9c376 |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-22T02:35:31Z |
| publishDate | 2014-11-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-2932002ecd794f39a4aa02cadec9c3762022-12-21T18:41:46ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502014-11-01291171171410.1590/S0102-86502014001800003Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivityTales Rubens de NadaiAna Paula Cassiano SilveiraAriadne Santana e Neves MonteiroDebora Ribeiro CamposMarco Tulio Rezende de CarvalhoAgnes Afrodite Sumarelli AlbuquerqueAndrea Carla CelottoPaulo Roberto Barbosa EvoraPURPOSE:To create in vitro a model to generate acidosis by CO2bubbling "organ chambers", which would be useful for researchers that aim to study the effects of acid-base disturbs on the endothelium-dependent vascular reactivity.METHODS:Eighteen male Wistar rats (230-280g) were housed, before the experiments, under standard laboratory conditions (12h light/dark cycle at 21°C), with free access to food and water. The protocol for promoting in vitro respiratory acidosis was carried out by bubbling increased concentrations of CO2. The target was to achieve an ideal way to decrease the pH gradually to a value of approximately 6.6.It was used, initially, a gas blender varying concentrations of the carbogenic mixture (95% O2 + 5% CO2) and pure CO2.RESULTS:1) 100% CO2, pH variation very fast, pH minimum 6.0; 2) 90%CO2 pH variation bit slower, pH minimum6.31; 3) 70%CO2, pH variation slower, pH minimum 6.32; 4) 50% CO2, pH variation slower, pH minimum 6:42; 5) 40 %CO2, Adequate record, pH minimum 6.61, and; 6) 30 %CO2 could not reach values below pH minimum 7.03. Based on these data the gas mixture (O2 60% + CO2 40%) was adopted,CONCLUSION: This gas mixture (O2 60% + CO2 40%) was effective in inducing respiratory acidosis at a speed that made, possible the recording of isometric force.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001100711&tlng=enAcidosis, RespiratoryEndotheliumNitric OxideRats |
| spellingShingle | Tales Rubens de Nadai Ana Paula Cassiano Silveira Ariadne Santana e Neves Monteiro Debora Ribeiro Campos Marco Tulio Rezende de Carvalho Agnes Afrodite Sumarelli Albuquerque Andrea Carla Celotto Paulo Roberto Barbosa Evora Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity Acta Cirúrgica Brasileira Acidosis, Respiratory Endothelium Nitric Oxide Rats |
| title | Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity |
| title_full | Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity |
| title_fullStr | Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity |
| title_full_unstemmed | Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity |
| title_short | Presentation of an experimental method to induce in vitro ("organ chambers") respiratory acidosis and its effect on vascular reactivity |
| title_sort | presentation of an experimental method to induce in vitro organ chambers respiratory acidosis and its effect on vascular reactivity |
| topic | Acidosis, Respiratory Endothelium Nitric Oxide Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001100711&tlng=en |
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