The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine
Pickering emulsions were prepared by phacoemulsification in an ice water bath with squalene as the oil phase and an aluminum adjuvant as the particle stabilizer. The effects of formulation and process conditions on the size and distribution of the Pickering emulsions were investigated. Pickering emu...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-07-01
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| Series: | ChemPhysMater |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772571523000220 |
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| author | Zhuanqing Huang Qi Sun Haoyuan Shi Sen Yang Yuanyuan Li Yue Ma Fei Yang Zhenwei Shi Yalong Yang Ying Zhang Hui Gong Fenghua Xu |
| author_facet | Zhuanqing Huang Qi Sun Haoyuan Shi Sen Yang Yuanyuan Li Yue Ma Fei Yang Zhenwei Shi Yalong Yang Ying Zhang Hui Gong Fenghua Xu |
| author_sort | Zhuanqing Huang |
| collection | DOAJ |
| description | Pickering emulsions were prepared by phacoemulsification in an ice water bath with squalene as the oil phase and an aluminum adjuvant as the particle stabilizer. The effects of formulation and process conditions on the size and distribution of the Pickering emulsions were investigated. Pickering emulsions prepared under the optimal prescription and process conditions were mixed with a peptide antigen to obtain a peptide vaccine. The optimal prescription and process condition of the Pickering emulsion is as follows: squalene as the oil phase, ultra-pure water as the water phase with 5 mg/mL aluminum adjuvant, and an ultrasonication time of 4 min at 200 W power. BALB/c mice were immunized with the peptide vaccine, and the ability of the Pickering emulsion as an immunological adjuvant to improve the efficacy of the peptide vaccine was evaluated. Under optimal conditions, a Pickering emulsion with a small particle size (430.8 nm), uniform distribution (polydispersion index of 16.9%), and zeta potential of 31.5 mV, was obtained. Immunological results showed that the serum specific antibody level in the vaccinated group reached 1×104 after three immunizations. The proportion of CD4+T cells and CD4/CD8 cells was significantly higher (P<0.05) in the vaccinated groups than the blank control group. Further, cytokine (TNF-α) secretion decreased in the aluminum adjuvant and Pickering emulsion groups but increased in the Freund's adjuvant group. All three vaccinated groups of mice exhibited low but detectable levels of IFN-γ secretion. |
| first_indexed | 2024-03-12T23:04:27Z |
| format | Article |
| id | doaj.art-2935e3635db14ac2b0095ba0a7cdfa45 |
| institution | Directory Open Access Journal |
| issn | 2772-5715 |
| language | English |
| last_indexed | 2024-03-12T23:04:27Z |
| publishDate | 2023-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | ChemPhysMater |
| spelling | doaj.art-2935e3635db14ac2b0095ba0a7cdfa452023-07-19T04:24:05ZengKeAi Communications Co., Ltd.ChemPhysMater2772-57152023-07-0123259266The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccineZhuanqing Huang0Qi Sun1Haoyuan Shi2Sen Yang3Yuanyuan Li4Yue Ma5Fei Yang6Zhenwei Shi7Yalong Yang8Ying Zhang9Hui Gong10Fenghua Xu11Pharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaMedical School of Chinese PLA, Beijing 100853, China; Chinese People's Armed Police Force Hospital of Beijing, Beijing 100600, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Medical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, ChinaMedical School of Chinese PLA, Beijing 100853, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre, PLA General Hospital, Beijing 100853, China; Corresponding author.Pickering emulsions were prepared by phacoemulsification in an ice water bath with squalene as the oil phase and an aluminum adjuvant as the particle stabilizer. The effects of formulation and process conditions on the size and distribution of the Pickering emulsions were investigated. Pickering emulsions prepared under the optimal prescription and process conditions were mixed with a peptide antigen to obtain a peptide vaccine. The optimal prescription and process condition of the Pickering emulsion is as follows: squalene as the oil phase, ultra-pure water as the water phase with 5 mg/mL aluminum adjuvant, and an ultrasonication time of 4 min at 200 W power. BALB/c mice were immunized with the peptide vaccine, and the ability of the Pickering emulsion as an immunological adjuvant to improve the efficacy of the peptide vaccine was evaluated. Under optimal conditions, a Pickering emulsion with a small particle size (430.8 nm), uniform distribution (polydispersion index of 16.9%), and zeta potential of 31.5 mV, was obtained. Immunological results showed that the serum specific antibody level in the vaccinated group reached 1×104 after three immunizations. The proportion of CD4+T cells and CD4/CD8 cells was significantly higher (P<0.05) in the vaccinated groups than the blank control group. Further, cytokine (TNF-α) secretion decreased in the aluminum adjuvant and Pickering emulsion groups but increased in the Freund's adjuvant group. All three vaccinated groups of mice exhibited low but detectable levels of IFN-γ secretion.http://www.sciencedirect.com/science/article/pii/S2772571523000220Pickering emulsionsUltrasonic emulsificationImmunologic adjuvantCOVID-19Polypeptide vaccine |
| spellingShingle | Zhuanqing Huang Qi Sun Haoyuan Shi Sen Yang Yuanyuan Li Yue Ma Fei Yang Zhenwei Shi Yalong Yang Ying Zhang Hui Gong Fenghua Xu The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine ChemPhysMater Pickering emulsions Ultrasonic emulsification Immunologic adjuvant COVID-19 Polypeptide vaccine |
| title | The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine |
| title_full | The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine |
| title_fullStr | The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine |
| title_full_unstemmed | The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine |
| title_short | The effect of Pickering emulsion adjuvants on the immune efficacy of the COVID-19 polypeptide vaccine |
| title_sort | effect of pickering emulsion adjuvants on the immune efficacy of the covid 19 polypeptide vaccine |
| topic | Pickering emulsions Ultrasonic emulsification Immunologic adjuvant COVID-19 Polypeptide vaccine |
| url | http://www.sciencedirect.com/science/article/pii/S2772571523000220 |
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