Development of a molecularly evolved, highly sensitive CaMKII FRET sensor with improved expression pattern.

Development of a molecularly evolved, highly sensitive CaMKII FRET sensor with improved expression pattern.

Genetically encoded fluorescence resonance energy transfer (FRET) biosensors have been successfully used to visualize protein activity in living cells. The sensitivity and accuracy of FRET measurements directly depend on biosensor folding efficiency, expression pattern, sensitivity, and dynamic rang...

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Bibliographic Details
Main Authors: Akihiro C E Shibata, Hiroshi K Maebashi, Yoshihisa Nakahata, Junichi Nabekura, Hideji Murakoshi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4370617?pdf=render

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http://europepmc.org/articles/PMC4370617?pdf=render

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