Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins

Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins

Labeling of bacterial cells with fluorescent proteins allows tracking the bacteria in competition and interactomic in vivo and in vitro studies. During the last years, a few plasmid vectors have been developed aimed at the fluorescent labeling of specific members of the lactic acid bacteria (LAB), a...

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Bibliographic Details
Main Authors: Javier Nicolás Garay-Novillo, Diego García-Morena, José Ángel Ruiz-Masó, José Luis Barra, Gloria del Solar
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-06-01
Series:Frontiers in Microbiology
Subjects:
lactic acid bacteria
fluorescent labeling vectors
plasmid copy number
plasmid stability
plasmid fitness cost
mCherry and EGFP
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01431/full

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https://www.frontiersin.org/article/10.3389/fmicb.2019.01431/full

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