Bee bread - perspective source of bioactive compounds for future

<table style="height: 589px;" border="0" cellspacing="0" cellpadding="0" width="664" align="left"><tbody><tr><td height="589" align="left" valign="top"><p>Bee bread is product wi...

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Main Authors: Eva Ivanišová, Miroslava Kačániová, Helena Frančáková, Jana Petrová, Jana Hutková, Valeryii Brovarskyi, Serhii Velychko, Leonora Adamchuk, Zuzana Schubertová, Janette Musilová
Format: Article
Language:English
Published: HACCP Consulting 2015-12-01
Series:Potravinarstvo
Subjects:
Online Access:http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/558
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author Eva Ivanišová
Miroslava Kačániová
Helena Frančáková
Jana Petrová
Jana Hutková
Valeryii Brovarskyi
Serhii Velychko
Leonora Adamchuk
Zuzana Schubertová
Janette Musilová
author_facet Eva Ivanišová
Miroslava Kačániová
Helena Frančáková
Jana Petrová
Jana Hutková
Valeryii Brovarskyi
Serhii Velychko
Leonora Adamchuk
Zuzana Schubertová
Janette Musilová
author_sort Eva Ivanišová
collection DOAJ
description <table style="height: 589px;" border="0" cellspacing="0" cellpadding="0" width="664" align="left"><tbody><tr><td height="589" align="left" valign="top"><p>Bee bread is product with long history used mainly in folk medicine. Nowadays, bee bread is growing in commercial interest due to its high nutritional properties. The objective of this study was to determine biological activity of ethanolic extract of bee bread obtained from selected region of Ukraine - Poltava oblast, Kirovohrad oblast, Vinnica oblast, Kyiv oblast, Dnepropetrovsk oblast. The antioxidant activity was measured with the radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as phosphomolybdenum assay. Total polyphenol content was determined with Folin-Ciocalteau reagent and total flavonoid content by aluminium-chloride method. Secondary was also evaluated antimicrobial activity in bee bread samples with disc diffusion method and <em>minimum inhibitory concentrations</em>. Antioxidant activity expressed as mg TEAC per g of dry weight (Trolox equivalent antioxidant capacity) was the highest in bee bread from Poltava oblast in DPPH and also phosphomolybdenum method. Samples of bee bread contained high levels of total polyphenols (12.36 - 18.24 mg GAE - gallic acid equivalent per g of dry weight) and flavonoids (13.56 - 18.24 &mu;g QE - quercetin equivalent per g of dry weight) with the best values of bee bread from Poltava oblast. An elevated level of antioxidant potential in the bee bread determines its biological properties, which conditioned of the biological active substances. The best antibacterial activity of bee bred with disc diffusion method was found against <em>Bacillus thuringiensis</em> CCM 19. The antibacterial activity inhibited by the bee bread extract in the present study indicate that best minimal inhibition concentration was against bacteria <em>Escherichia coli</em> CCM 3988 and <em>Salmonella enterica</em> subs. <em>enterica</em> CCM 3807.</p></td></tr></tbody></table> <!--[endif] -->
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spelling doaj.art-29809c4c6e5b4e21b30acc8a195263142022-12-21T21:43:19ZengHACCP ConsultingPotravinarstvo1337-09602015-12-019159259810.5219/558403Bee bread - perspective source of bioactive compounds for futureEva Ivanišová0Miroslava Kačániová1Helena Frančáková2Jana Petrová3Jana Hutková4Valeryii Brovarskyi5Serhii Velychko6Leonora Adamchuk7Zuzana Schubertová8Janette Musilová9Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Plant Storage and Processing, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra , Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Plant Storage and Processing, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra , Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra , Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 NitraNational University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine, Department of beekeeping, Heroiv Oborony St, 15, Kiev, Ukrajina, 03041.National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine, Department of beekeeping, Heroiv Oborony St, 15, Kiev, 03041National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine, Department of beekeeping, Heroiv Oborony St, 15, Kiev, 03041Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Reources, Institute of Biodiversity and Biological Safety, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra<table style="height: 589px;" border="0" cellspacing="0" cellpadding="0" width="664" align="left"><tbody><tr><td height="589" align="left" valign="top"><p>Bee bread is product with long history used mainly in folk medicine. Nowadays, bee bread is growing in commercial interest due to its high nutritional properties. The objective of this study was to determine biological activity of ethanolic extract of bee bread obtained from selected region of Ukraine - Poltava oblast, Kirovohrad oblast, Vinnica oblast, Kyiv oblast, Dnepropetrovsk oblast. The antioxidant activity was measured with the radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as phosphomolybdenum assay. Total polyphenol content was determined with Folin-Ciocalteau reagent and total flavonoid content by aluminium-chloride method. Secondary was also evaluated antimicrobial activity in bee bread samples with disc diffusion method and <em>minimum inhibitory concentrations</em>. Antioxidant activity expressed as mg TEAC per g of dry weight (Trolox equivalent antioxidant capacity) was the highest in bee bread from Poltava oblast in DPPH and also phosphomolybdenum method. Samples of bee bread contained high levels of total polyphenols (12.36 - 18.24 mg GAE - gallic acid equivalent per g of dry weight) and flavonoids (13.56 - 18.24 &mu;g QE - quercetin equivalent per g of dry weight) with the best values of bee bread from Poltava oblast. An elevated level of antioxidant potential in the bee bread determines its biological properties, which conditioned of the biological active substances. The best antibacterial activity of bee bred with disc diffusion method was found against <em>Bacillus thuringiensis</em> CCM 19. The antibacterial activity inhibited by the bee bread extract in the present study indicate that best minimal inhibition concentration was against bacteria <em>Escherichia coli</em> CCM 3988 and <em>Salmonella enterica</em> subs. <em>enterica</em> CCM 3807.</p></td></tr></tbody></table> <!--[endif] -->http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/558antioxidant activitypollenflavonoidspolyphenolsantimicrobial activity
spellingShingle Eva Ivanišová
Miroslava Kačániová
Helena Frančáková
Jana Petrová
Jana Hutková
Valeryii Brovarskyi
Serhii Velychko
Leonora Adamchuk
Zuzana Schubertová
Janette Musilová
Bee bread - perspective source of bioactive compounds for future
Potravinarstvo
antioxidant activity
pollen
flavonoids
polyphenols
antimicrobial activity
title Bee bread - perspective source of bioactive compounds for future
title_full Bee bread - perspective source of bioactive compounds for future
title_fullStr Bee bread - perspective source of bioactive compounds for future
title_full_unstemmed Bee bread - perspective source of bioactive compounds for future
title_short Bee bread - perspective source of bioactive compounds for future
title_sort bee bread perspective source of bioactive compounds for future
topic antioxidant activity
pollen
flavonoids
polyphenols
antimicrobial activity
url http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/558
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