Application of In Situ PCR to Yeast Cells for Screening YAC Libraries
We have used in situ PCR technology in yeast cells with the ultimate goal of cloning and screening genomic yeast artificial chromosome (YAC) libraries. The target sequences in YAC clones were amplified “in situ” in yeast cells by the same set of microsatellite primers used in solution-based PCR scre...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99273st06 |