Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications

Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications

Ultra-deep sequencing (UDS) of amplicons is a major application for next-generation sequencing tech nologies, even more so for the 454 Genome Sequencer FLX. Especially for this application, errors that might be introduced during any of the sample processing or data analysis steps should be avoided o...

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Bibliographic Details
Main Authors: Ina Vandenbroucke, Herwig Van Marck, Peter Verhasselt, Kim Thys, Wendy Mostmans, Stéphanie Dumont, Veerle Van Eygen, Katrien Coen, Marianne Tuefferd, Jeroen Aerssens
Format: Article
Language:English
Published: Taylor & Francis Group 2011-09-01
Series:BioTechniques
Subjects:
ultra-deep sequencing
454 pyrosequencing
minor variant detection
DNA polymerase
sequencing error
Online Access:https://www.future-science.com/doi/10.2144/000113733

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https://www.future-science.com/doi/10.2144/000113733

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