Extracellular vesicle depletion and UGCG overexpression mitigate the cell density effect in HEK293 cell culture transfection

Extracellular vesicle depletion and UGCG overexpression mitigate the cell density effect in HEK293 cell culture transfection

The hitherto unexplained reduction of cell-specific productivity in transient gene expression (TGE) at high cell density (HCD) is known as the cell density effect (CDE). It currently represents a major challenge in TGE-based bioprocess intensification. This phenomenon has been largely reported, but...

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Bibliographic Details
Main Authors: Pol Pérez-Rubio, Jesús Lavado-García, Laia Bosch-Molist, Elianet Lorenzo Romero, Laura Cervera, Francesc Gòdia
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
cell density effect
extracellular vesicles
VLP
transient gene expression
HEK293
cell culture
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050124000068

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http://www.sciencedirect.com/science/article/pii/S2329050124000068

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