Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering. We hyphenated FcɣRIIIa affinity chromatography to mass spectrometry, which allowed direct affini...

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Bibliographic Details
Main Authors: Steffen Lippold, Simone Nicolardi, Elena Domínguez-Vega, Anna-Katharina Heidenreich, Gestur Vidarsson, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, David Falck
Format: Article
Language:English
Published: Taylor & Francis Group 2019-10-01
Series:mAbs
Subjects:
Monoclonal antibody (mAb)
FcɣRIIIa affinity chromatography
IgG Fc glycosylation
immunoglobulin glycans
mass spectrometry
FT-ICR-MS
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2019.1636602

Internet

https://www.tandfonline.com/doi/10.1080/19420862.2019.1636602

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