CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles

Abstract Background The application of CRISPR/Cas9 technology in human induced pluripotent stem cells (hiPSC) holds tremendous potential for basic research and cell-based gene therapy. However, the fulfillment of these promises relies on the capacity to efficiently deliver exogenous nucleic acids an...

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Bibliographic Details
Main Authors: Joffrey Mianné, Amel Nasri, Chloé Nguyen Van, Chloé Bourguignon, Mathieu Fieldès, Engi Ahmed, Christine Duthoit, Nicolas Martin, Hugues Parrinello, Anaïs Louis, Alexandra Iché, Régis Gayon, Florine Samain, Lucille Lamouroux, Pascale Bouillé, Arnaud Bourdin, Said Assou, John De Vos
Format: Article
Language:English
Published: BMC 2022-01-01
Series:BMC Biology
Subjects:
hiPSC
Transduction
CRISPR
Retrovirus-like particles
Gene conversion
Knock-out
Online Access:https://doi.org/10.1186/s12915-021-01214-x

Internet

https://doi.org/10.1186/s12915-021-01214-x

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