Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats
Background: Moonseed vine (Triclisia gilletii Staner) a member of the Menispermaceae family, has been previously investigated and reported in our laboratory to exhibit antilithiatic potentials against ethane-1,2-diol induced nephrolithiasis. However, the mechanism underlying its action is not clear....
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| Language: | English |
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Elsevier
2021-11-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031321000488 |
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| author | Olanrewaju Sam Olayeriju Olusola Olalekan Elekofehinti Mary Tolulope Olaleye Akintunde Afolabi Akindahunsi |
| author_facet | Olanrewaju Sam Olayeriju Olusola Olalekan Elekofehinti Mary Tolulope Olaleye Akintunde Afolabi Akindahunsi |
| author_sort | Olanrewaju Sam Olayeriju |
| collection | DOAJ |
| description | Background: Moonseed vine (Triclisia gilletii Staner) a member of the Menispermaceae family, has been previously investigated and reported in our laboratory to exhibit antilithiatic potentials against ethane-1,2-diol induced nephrolithiasis. However, the mechanism underlying its action is not clear. Purpose: Mechanism of action of aqueous methanolic leaf extact of Triclisia gilletii (TGAMLE 100 mg/kg) in comparison with compounds identified in TGAMLE (Quercetin (20 mg/kg), oleanolic acid (10 mg/kg), stigmasterol (20 mg/kg), and sitosterol (20 mg/kg)) was investigated against ethane-1,2-diol administered rats. Methods: The mRNA expression of antioxidant marker genes (nuclear factor erythroid- 2 – related factor- 2 (NRF2) and Heme oxygenase-1 (HO-1)) and crystal binding genes (CD44 and osteopontin (OPN)) were assessed using RT-PCR. Results: Ethane-1,2-diol administration down-regulated antioxidant marker genes (NRF2 and HO-1) and up-regulated mRNA expression of CD44 with no significant difference in OPN when compared with control. TGAMLE and its derived compounds significantly activated the NRF2/HO-1 pathway by up-regulating its expression and modify crystal binding molecules (CD44/OPN). Overall, the additive effects of the compounds present in the extract revealed a better efficacy in attenuating NRF2/HO-1 pathway as well as the expression of crystal binding molecules. Conclusion: The present study concludes the nephro-protective effect and underlying mechanism of TGAMLE against ethane-1,2-diol exposed rats and suggests that TGAMLE or compounds in TGAMLE could be an alternative agent against kidney stones. |
| first_indexed | 2024-12-14T08:10:30Z |
| format | Article |
| id | doaj.art-29e0149634424c32913b8506fcd67997 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-12-14T08:10:30Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-29e0149634424c32913b8506fcd679972022-12-21T23:10:04ZengElsevierPhytomedicine Plus2667-03132021-11-0114100066Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic ratsOlanrewaju Sam Olayeriju0Olusola Olalekan Elekofehinti1Mary Tolulope Olaleye2Akintunde Afolabi Akindahunsi3Department of Chemical Sciences (Biochemistry Unit) Olusegun Agagu University of Science and Technology Okitipupa, Nigeria; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, P.M.B 704, Nigeria; Phytomedicine and Toxicology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, P.M.B 704, Nigeria; Corresponding author.Bioinformatics and Molecular Biology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, P.M.B 704, NigeriaPhytomedicine and Toxicology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, P.M.B 704, NigeriaPhytomedicine and Toxicology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, P.M.B 704, NigeriaBackground: Moonseed vine (Triclisia gilletii Staner) a member of the Menispermaceae family, has been previously investigated and reported in our laboratory to exhibit antilithiatic potentials against ethane-1,2-diol induced nephrolithiasis. However, the mechanism underlying its action is not clear. Purpose: Mechanism of action of aqueous methanolic leaf extact of Triclisia gilletii (TGAMLE 100 mg/kg) in comparison with compounds identified in TGAMLE (Quercetin (20 mg/kg), oleanolic acid (10 mg/kg), stigmasterol (20 mg/kg), and sitosterol (20 mg/kg)) was investigated against ethane-1,2-diol administered rats. Methods: The mRNA expression of antioxidant marker genes (nuclear factor erythroid- 2 – related factor- 2 (NRF2) and Heme oxygenase-1 (HO-1)) and crystal binding genes (CD44 and osteopontin (OPN)) were assessed using RT-PCR. Results: Ethane-1,2-diol administration down-regulated antioxidant marker genes (NRF2 and HO-1) and up-regulated mRNA expression of CD44 with no significant difference in OPN when compared with control. TGAMLE and its derived compounds significantly activated the NRF2/HO-1 pathway by up-regulating its expression and modify crystal binding molecules (CD44/OPN). Overall, the additive effects of the compounds present in the extract revealed a better efficacy in attenuating NRF2/HO-1 pathway as well as the expression of crystal binding molecules. Conclusion: The present study concludes the nephro-protective effect and underlying mechanism of TGAMLE against ethane-1,2-diol exposed rats and suggests that TGAMLE or compounds in TGAMLE could be an alternative agent against kidney stones.http://www.sciencedirect.com/science/article/pii/S2667031321000488AntilithiaticNRF2/HO-1 PathwayTriclisia gilletiiethane-1,2-diolNephrolithiasisCrystal binding genes |
| spellingShingle | Olanrewaju Sam Olayeriju Olusola Olalekan Elekofehinti Mary Tolulope Olaleye Akintunde Afolabi Akindahunsi Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats Phytomedicine Plus Antilithiatic NRF2/HO-1 Pathway Triclisia gilletii ethane-1,2-diol Nephrolithiasis Crystal binding genes |
| title | Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats |
| title_full | Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats |
| title_fullStr | Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats |
| title_full_unstemmed | Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats |
| title_short | Activation of NRF2/HO-1 Pathway by aqueous methanolic leaf extract of Triclisia gilletii and selected identified compounds in Triclisia gilletii, modulates crystal binding genes (CD44/OPN) in Ethane-1,2-diol-induced nephrolithic rats |
| title_sort | activation of nrf2 ho 1 pathway by aqueous methanolic leaf extract of triclisia gilletii and selected identified compounds in triclisia gilletii modulates crystal binding genes cd44 opn in ethane 1 2 diol induced nephrolithic rats |
| topic | Antilithiatic NRF2/HO-1 Pathway Triclisia gilletii ethane-1,2-diol Nephrolithiasis Crystal binding genes |
| url | http://www.sciencedirect.com/science/article/pii/S2667031321000488 |
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