Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts
Herein, we isolated three triterpenoid saponins from the methanol extract of the fruit pulp of argan. The structures of the identified compounds were determined using comprehensive NMR spectroscopy analyses (1H, 13C NMR, COSY, TOCSY, ROESY, and HSQC), combined with mass spectroscopy. Gas chromatogra...
| Main Authors: | , , , , , |
|---|---|
| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
Elsevier
2024-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Acceso en liña: | http://www.sciencedirect.com/science/article/pii/S2405844023108206 |
| _version_ | 1827367209743155200 |
|---|---|
| author | Asmae Alaoui Nihad Sahri Ismail Mahdi Nidal Fahsi El hassania El herradi Mansour Sobeh |
| author_facet | Asmae Alaoui Nihad Sahri Ismail Mahdi Nidal Fahsi El hassania El herradi Mansour Sobeh |
| author_sort | Asmae Alaoui |
| collection | DOAJ |
| description | Herein, we isolated three triterpenoid saponins from the methanol extract of the fruit pulp of argan. The structures of the identified compounds were determined using comprehensive NMR spectroscopy analyses (1H, 13C NMR, COSY, TOCSY, ROESY, and HSQC), combined with mass spectroscopy. Gas chromatography (GC) was utilized to determine the monosaccharide contents after the samples underwent methanolysis and their glycoside configuration was proved via their trimethylsilyl derivatives. Furthermore, the methanol extract of the fruit pulp and its n-butanol fraction were evaluated for their antioxidant properties via DPPH, ABTS, and FRAP assays, antidiabetic activity using α-amylase and α-glucosidase inhibition activities, and antibacterial properties utilizing microdilution and antibiofilm assays. Compared to the crude methanol extract, our results showed that the n-butanol fraction exhibited more potent antioxidant activity and antibacterial potential against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Enterococcus faecalis, and Pseudomonas aeruginosa (MIC = 12.5–50 mg/mL); while no effect on the bacterial biofilm was observed. The methanol extract was more effective in inhibiting α-glucosidase (EC50 = 0.15 mg/mL), however, the n-butanol fraction elicited strong α-amylase inhibition (EC50 = 0.49 mg/mL). These findings suggest that the fruit pulp of argan could serve as a potential source of phytochemicals suitable for the treatment of diabetes and its related complications. |
| first_indexed | 2024-03-08T09:02:16Z |
| format | Article |
| id | doaj.art-29fb47ef152e4327a5df8e01c7cef286 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:02:16Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-29fb47ef152e4327a5df8e01c7cef2862024-02-01T06:32:38ZengElsevierHeliyon2405-84402024-01-01101e23612Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extractsAsmae Alaoui0Nihad Sahri1Ismail Mahdi2Nidal Fahsi3El hassania El herradi4Mansour Sobeh5Physio-Chemical Laboratory of Inorganic and Organic Materials (LPCMIO), Materials Science Center (MSC), Ecole Normale Supérieure, Mohammed V University in Rabat, Rabat, Morocco; Corresponding author.Physio-Chemical Laboratory of Inorganic and Organic Materials (LPCMIO), Materials Science Center (MSC), Ecole Normale Supérieure, Mohammed V University in Rabat, Rabat, Morocco; AgroBioSciences Program, College of Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir 43150, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir 43150, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir 43150, MoroccoPhysio-Chemical Laboratory of Inorganic and Organic Materials (LPCMIO), Materials Science Center (MSC), Ecole Normale Supérieure, Mohammed V University in Rabat, Rabat, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir 43150, Morocco; Corresponding author.Herein, we isolated three triterpenoid saponins from the methanol extract of the fruit pulp of argan. The structures of the identified compounds were determined using comprehensive NMR spectroscopy analyses (1H, 13C NMR, COSY, TOCSY, ROESY, and HSQC), combined with mass spectroscopy. Gas chromatography (GC) was utilized to determine the monosaccharide contents after the samples underwent methanolysis and their glycoside configuration was proved via their trimethylsilyl derivatives. Furthermore, the methanol extract of the fruit pulp and its n-butanol fraction were evaluated for their antioxidant properties via DPPH, ABTS, and FRAP assays, antidiabetic activity using α-amylase and α-glucosidase inhibition activities, and antibacterial properties utilizing microdilution and antibiofilm assays. Compared to the crude methanol extract, our results showed that the n-butanol fraction exhibited more potent antioxidant activity and antibacterial potential against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Enterococcus faecalis, and Pseudomonas aeruginosa (MIC = 12.5–50 mg/mL); while no effect on the bacterial biofilm was observed. The methanol extract was more effective in inhibiting α-glucosidase (EC50 = 0.15 mg/mL), however, the n-butanol fraction elicited strong α-amylase inhibition (EC50 = 0.49 mg/mL). These findings suggest that the fruit pulp of argan could serve as a potential source of phytochemicals suitable for the treatment of diabetes and its related complications.http://www.sciencedirect.com/science/article/pii/S2405844023108206ArganPulpTriterpenoid saponinsAntioxidant activityAntidiabetic activityAntibacterial activity |
| spellingShingle | Asmae Alaoui Nihad Sahri Ismail Mahdi Nidal Fahsi El hassania El herradi Mansour Sobeh Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts Heliyon Argan Pulp Triterpenoid saponins Antioxidant activity Antidiabetic activity Antibacterial activity |
| title | Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts |
| title_full | Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts |
| title_fullStr | Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts |
| title_full_unstemmed | Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts |
| title_short | Argan: Phytochemical profiling and evaluation of the antioxidant, hypoglycemic, and antibacterial properties of its fruit pulp extracts |
| title_sort | argan phytochemical profiling and evaluation of the antioxidant hypoglycemic and antibacterial properties of its fruit pulp extracts |
| topic | Argan Pulp Triterpenoid saponins Antioxidant activity Antidiabetic activity Antibacterial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023108206 |
| work_keys_str_mv | AT asmaealaoui arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts AT nihadsahri arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts AT ismailmahdi arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts AT nidalfahsi arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts AT elhassaniaelherradi arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts AT mansoursobeh arganphytochemicalprofilingandevaluationoftheantioxidanthypoglycemicandantibacterialpropertiesofitsfruitpulpextracts |