Acceleration of CRISPR/Cas9-Mediated Editing at Multiple Sites in the <i>Saccharomyces cerevisiae</i> Genome
The application of the CRISPR/Cas9-based genome editing technique to the yeast <i>Saccharomyces cerevisiae</i> has made it possible to simultaneously modify several sites, particularly to integrate several expression cassettes. The existing methods provide high efficiency for such modifi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
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| Series: | Methods and Protocols |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2409-9279/6/2/39 |