Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Abstract Background The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to secretion. Simultaneous disruption of one va...

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Bibliographic Details
Main Authors: Lukas Marsalek, Verena Puxbaum, Markus Buchetics, Diethard Mattanovich, Brigitte Gasser
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Microbial Cell Factories
Subjects:
Komagataella
Vacuolar protein sorting
Recombinant protein production
Yeast
Protein secretion
Secretion enhancing factor
Online Access:http://link.springer.com/article/10.1186/s12934-019-1155-4

Internet

http://link.springer.com/article/10.1186/s12934-019-1155-4

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