Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit
Abstract Background Guava fruit softening is a crucial process during ripening and this process involves a number of enzymes that modifies the cell wall. Two of the enzymes that regulate this process are (a) the β-1, 4-endoglucanase 17 (BEG) which hydrolyze β-1, 4 bonds from cellulose and hemicellul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-021-00289-x |
| _version_ | 1797203966479564800 |
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| author | Mario A. Mejía-Mendoza Cristina Garcidueñas-Piña José S. Padilla-Ramírez Ruth E. Soria-Guerra José Francisco Morales-Domínguez |
| author_facet | Mario A. Mejía-Mendoza Cristina Garcidueñas-Piña José S. Padilla-Ramírez Ruth E. Soria-Guerra José Francisco Morales-Domínguez |
| author_sort | Mario A. Mejía-Mendoza |
| collection | DOAJ |
| description | Abstract Background Guava fruit softening is a crucial process during ripening and this process involves a number of enzymes that modifies the cell wall. Two of the enzymes that regulate this process are (a) the β-1, 4-endoglucanase 17 (BEG) which hydrolyze β-1, 4 bonds from cellulose and hemicellulose, and (b) β-galactosidase (BGA) that hydrolyzes pectin chains. Bioinformatics and expression analysis information on these genes is limited in guava fruit. Results A fragment of a β-1, 4-endoglucanase 17 (PgE17), and another of a β-galactosidase (PgGa1) were identified. These sequences have a similarity of more than 85% with those reported in the NCBI database. In the guava genome, one homologous sequence was found for PgE17 in Chr 4 and two homologous to PgGa1: one in Chr 3 and the other one in Chr 6. Putative protein PgE17 contains part of the glyco_hydro_9 domain. Putative protein PgGa1 has a part of the glyco_hydro_35 domain. Phylogenetic analysis of PgE17 and PgGa1 revealed that both are highly conserved inside the Myrtaceae family. In silico expression analysis showed that both PgE17 and PgGa1 work in a coordinated way with other cell wall modifier enzymes. Expression of these genes was found in all the guava samples analyzed. However, the highest expression was found in the fruit in the breaking and ripe states. Conclusions A β-1, 4-endoglucanase 17, and β-galactosidase 1 sequences were identified. PgE17 and PgGa1 are expressed in all the plant tissues, and fruit ripening states. Although, the highest expression was on breaker and ripe states. |
| first_indexed | 2024-04-24T08:27:44Z |
| format | Article |
| id | doaj.art-2a2b79f3f1684cd8ba05655a076e4db3 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:27:44Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-2a2b79f3f1684cd8ba05655a076e4db32024-04-16T21:18:50ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-01-0120111110.1186/s43141-021-00289-xIdentification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruitMario A. Mejía-Mendoza0Cristina Garcidueñas-Piña1José S. Padilla-Ramírez2Ruth E. Soria-Guerra3José Francisco Morales-Domínguez4Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes (UAA)Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes (UAA)Instituto Nacional de Investigaciones ForestalesFacultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí (UASLP)Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes (UAA)Abstract Background Guava fruit softening is a crucial process during ripening and this process involves a number of enzymes that modifies the cell wall. Two of the enzymes that regulate this process are (a) the β-1, 4-endoglucanase 17 (BEG) which hydrolyze β-1, 4 bonds from cellulose and hemicellulose, and (b) β-galactosidase (BGA) that hydrolyzes pectin chains. Bioinformatics and expression analysis information on these genes is limited in guava fruit. Results A fragment of a β-1, 4-endoglucanase 17 (PgE17), and another of a β-galactosidase (PgGa1) were identified. These sequences have a similarity of more than 85% with those reported in the NCBI database. In the guava genome, one homologous sequence was found for PgE17 in Chr 4 and two homologous to PgGa1: one in Chr 3 and the other one in Chr 6. Putative protein PgE17 contains part of the glyco_hydro_9 domain. Putative protein PgGa1 has a part of the glyco_hydro_35 domain. Phylogenetic analysis of PgE17 and PgGa1 revealed that both are highly conserved inside the Myrtaceae family. In silico expression analysis showed that both PgE17 and PgGa1 work in a coordinated way with other cell wall modifier enzymes. Expression of these genes was found in all the guava samples analyzed. However, the highest expression was found in the fruit in the breaking and ripe states. Conclusions A β-1, 4-endoglucanase 17, and β-galactosidase 1 sequences were identified. PgE17 and PgGa1 are expressed in all the plant tissues, and fruit ripening states. Although, the highest expression was on breaker and ripe states.https://doi.org/10.1186/s43141-021-00289-xPsidium guajavaCell wallExpression analysisBioinformatic analysisCell elongationFruit softening |
| spellingShingle | Mario A. Mejía-Mendoza Cristina Garcidueñas-Piña José S. Padilla-Ramírez Ruth E. Soria-Guerra José Francisco Morales-Domínguez Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit Journal of Genetic Engineering and Biotechnology Psidium guajava Cell wall Expression analysis Bioinformatic analysis Cell elongation Fruit softening |
| title | Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit |
| title_full | Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit |
| title_fullStr | Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit |
| title_full_unstemmed | Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit |
| title_short | Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit |
| title_sort | identification in silico and expression analysis of a β 1 4 endoglucanase and β galactosidase genes related to ripening in guava fruit |
| topic | Psidium guajava Cell wall Expression analysis Bioinformatic analysis Cell elongation Fruit softening |
| url | https://doi.org/10.1186/s43141-021-00289-x |
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