Size-selection of cDNA libraries for the cloning of cDNAs after suppression subtractive hybridization

Here we describe the establishment of size-selected cDNA libraries for the cloning of full-length cDNAs that were initially identified by suppression subtractive hybridization (SSH) technology as being differentially expressed. First, the SSH-cDNA fragments were used as 32P-probes to verify their le...

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Váldodahkkit: V. Lévesque, T. Fayad, K. Ndiaye, M. Nahé Diouf, J.G. Lussier
Materiálatiipa: Artihkal
Giella:English
Almmustuhtton: Taylor & Francis Group 2003-07-01
Ráidu:BioTechniques
Liŋkkat:https://www.future-science.com/doi/10.2144/03351st02