Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas
The present paper reports the determination of the activation energies and the optimum temperatures of starch hydrolysis by porcine pancreas <i>α</i>-amylase. The parameters were estimated based on the literature data on the activity curves versus temperature for starch hydrolysis by α-a...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/14/4117 |
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| author | Justyna Miłek |
| author_facet | Justyna Miłek |
| author_sort | Justyna Miłek |
| collection | DOAJ |
| description | The present paper reports the determination of the activation energies and the optimum temperatures of starch hydrolysis by porcine pancreas <i>α</i>-amylase. The parameters were estimated based on the literature data on the activity curves versus temperature for starch hydrolysis by α-amylase from porcine pancreas. It was assumed that both the hydrolysis reaction process and the deactivation process of <i>α</i>-amylase were first-order reactions by the enzyme concentration. A mathematical model describing the effect of temperature on porcine pancreas <i>α</i>-amylase activity was used. The determine deactivation energies <i>E<sub>a</sub></i> were from 19.82 ± 7.22 kJ/mol to 128.80 ± 9.27 kJ/mol, the obtained optimum temperatures <i>T<sub>opt</sub></i> were in the range from 311.06 ± 1.10 K to 326.52 ± 1.75 K. In turn, the values of deactivation energies <i>E<sub>d</sub></i> has been noted in the range from 123.57 ± 14.17 kJ/mol to 209.37 ± 5.17 kJ/mol. The present study is related to the starch hydrolysis by <i>α</i>-amylase. In the industry, the obtained results the values <i>E<sub>a</sub></i>, <i>E<sub>d</sub></i>, <i>T<sub>opt</sub></i> can be used to design and optimize starch hydrolysis by <i>α</i>-amylase porcine pancreas. The obtained results might also find application in research on the pharmaceutical preparations used to treat pancreatic insufficiency or prognosis of pancreatic cancer. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:31:14Z |
| publishDate | 2021-07-01 |
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| series | Molecules |
| spelling | doaj.art-2a3c6f998876403ea226a94b4aa5beff2023-11-22T04:28:49ZengMDPI AGMolecules1420-30492021-07-012614411710.3390/molecules26144117Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine PancreasJustyna Miłek0Department of Chemical and Biochemical Engineering, Faculty of Chemical Technology and Engineering, University of Science and Technology in Bydgoszcz, Seminaryjna 3, 85-326 Bydgoszcz, PolandThe present paper reports the determination of the activation energies and the optimum temperatures of starch hydrolysis by porcine pancreas <i>α</i>-amylase. The parameters were estimated based on the literature data on the activity curves versus temperature for starch hydrolysis by α-amylase from porcine pancreas. It was assumed that both the hydrolysis reaction process and the deactivation process of <i>α</i>-amylase were first-order reactions by the enzyme concentration. A mathematical model describing the effect of temperature on porcine pancreas <i>α</i>-amylase activity was used. The determine deactivation energies <i>E<sub>a</sub></i> were from 19.82 ± 7.22 kJ/mol to 128.80 ± 9.27 kJ/mol, the obtained optimum temperatures <i>T<sub>opt</sub></i> were in the range from 311.06 ± 1.10 K to 326.52 ± 1.75 K. In turn, the values of deactivation energies <i>E<sub>d</sub></i> has been noted in the range from 123.57 ± 14.17 kJ/mol to 209.37 ± 5.17 kJ/mol. The present study is related to the starch hydrolysis by <i>α</i>-amylase. In the industry, the obtained results the values <i>E<sub>a</sub></i>, <i>E<sub>d</sub></i>, <i>T<sub>opt</sub></i> can be used to design and optimize starch hydrolysis by <i>α</i>-amylase porcine pancreas. The obtained results might also find application in research on the pharmaceutical preparations used to treat pancreatic insufficiency or prognosis of pancreatic cancer.https://www.mdpi.com/1420-3049/26/14/4117porcine pancreas <i>α</i>-amylaseactivation energydeactivation energyoptimum temperature |
| spellingShingle | Justyna Miłek Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas Molecules porcine pancreas <i>α</i>-amylase activation energy deactivation energy optimum temperature |
| title | Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas |
| title_full | Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas |
| title_fullStr | Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas |
| title_full_unstemmed | Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas |
| title_short | Determination of Activation Energies and the Optimum Temperatures of Hydrolysis of Starch by <i>α</i>-Amylase from Porcine Pancreas |
| title_sort | determination of activation energies and the optimum temperatures of hydrolysis of starch by i α i amylase from porcine pancreas |
| topic | porcine pancreas <i>α</i>-amylase activation energy deactivation energy optimum temperature |
| url | https://www.mdpi.com/1420-3049/26/14/4117 |
| work_keys_str_mv | AT justynamiłek determinationofactivationenergiesandtheoptimumtemperaturesofhydrolysisofstarchbyiaiamylasefromporcinepancreas |