Maintenance of Functional Human Cancellous Bone and Human Hematopoiesis in NOD/SCID Mice
Attempts were made to establish models to study interactions between marrow stromal cells and hematopoietic cells in vivo. The approach was to create a NOD-SCID-hu murine model of long-term human hematopoiesis by implantation of a human adult bone fragment. Nine to 12 weeks posttransplantation, huma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2004-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000004783983387 |
| Summary: | Attempts were made to establish models to study interactions between marrow stromal cells and hematopoietic cells in vivo. The approach was to create a NOD-SCID-hu murine model of long-term human hematopoiesis by implantation of a human adult bone fragment. Nine to 12 weeks posttransplantation, human CD45+ cells were detected in the blood and the spleen of some mice. The histology of the human transplant showed that human bone fragment was viable at 9 weeks. Moreover, vessels of human origin, as assessed by immunohistochemical detection of human β 2 -microglobulin, were observed in the mouse tissue surrounding the transplanted human fragment. |
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| ISSN: | 0963-6897 1555-3892 |