A two-stage digestion of whole murine knee joints for single-cell RNA sequencing

A two-stage digestion of whole murine knee joints for single-cell RNA sequencing

Objective: Single-cell RNA sequencing (scRNA-seq) is a powerful technology that can be applied to the cells populating the whole knee in the study of joint pathology. The knee contains cells embedded in hard structural tissues, cells in softer tissues and membranes, and immune cells. This creates a...

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Bibliographic Details
Main Authors: Dustin M. Leale, Linan Li, Matthew L. Settles, Keith Mitchell, Lutz Froenicke, Jasper H.N. Yik, Dominik R. Haudenschild
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Osteoarthritis and Cartilage Open
Subjects:
Single-cell RNA sequencing
Osteoarthritis
Whole knee joint
Tissue cross-talk
Mouse model
Online Access:http://www.sciencedirect.com/science/article/pii/S2665913122000899

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http://www.sciencedirect.com/science/article/pii/S2665913122000899

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