GATS tag system is compatible with biotin labelling methods for protein analysis

Abstract Polypeptide tags and biotin labelling technologies are widely used for protein analyses in biochemistry and cell biology. However, many peptide tag epitopes contain lysine residues (or amino acids) that are masked after biotinylation. Here, we propose the GATS tag system without a lysine re...

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Bibliographic Details
Main Authors: Kohdai Yamada, Fumiya Soga, Soh Tokunaga, Hikaru Nagaoka, Tatsuhiko Ozawa, Hiroyuki Kishi, Eizo Takashima, Tatsuya Sawasaki
Format: Article
Language:English
Published: Nature Portfolio 2023-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-36858-y