Comprehensive analysis of transglutaminase substrate preference by cDNA display coupled with next-generation sequencing and bioinformatics

Abstract cDNA display is an in vitro display technology based on a covalent linkage between a protein and its corresponding mRNA/cDNA, widely used for the selection of proteins and peptides from large libraries (1012) in a high throughput manner, based on their binding affinity. Here, we developed a...

Full description

Bibliographic Details
Main Authors: Jasmina Damnjanović, Nana Odake, Jicheng Fan, Maurizio Camagna, Beixi Jia, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Hideo Nakano
Format: Article
Language:English
Published: Nature Portfolio 2022-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-17494-4