β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium
Several studies have implicated b2-microglobulin (B2M) in osteoarthritis (OA) pathology. Of the main constituents of synovial tissue, synovial fibroblasts and macrophages, the latter play a pivotal role in inflammation. Although several studies have investigated the effects of B2M on synovial fibrob...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2023-01-01
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| Series: | Central European Journal of Immunology |
| Subjects: | |
| Online Access: | https://www.termedia.pl/-2-microglobulin-alters-the-profiles-of-inflammatory-cytokines-and-of-matrix-metalloprotease-in-macrophages-derived-from-the-osteoarthritic-synovium,10,49898,1,1.html |
| _version_ | 1797842691695837184 |
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| author | Kyoko Muneshige Kentaro Uchida Jun Aikawa Dai Iwase Shotaro Takano Masayuki Miyagi Gen Inoue Masashi Takaso |
| author_facet | Kyoko Muneshige Kentaro Uchida Jun Aikawa Dai Iwase Shotaro Takano Masayuki Miyagi Gen Inoue Masashi Takaso |
| author_sort | Kyoko Muneshige |
| collection | DOAJ |
| description | Several studies have implicated b2-microglobulin (B2M) in osteoarthritis (OA) pathology. Of the main constituents of synovial tissue, synovial fibroblasts and macrophages, the latter play a pivotal role in inflammation. Although several studies have investigated the effects of B2M on synovial fibroblasts, few have examined the impact on synovial macrophages. Here, we investigated the effect of B2M on the expression profiles of inflammatory cytokines and matrix metalloproteases (MMPs) in synovial macrophages.
Synovial macrophages were isolated from the osteoarthritic synovium using an anti-CD14 anti- body and magnetic isolation system. Synovial macrophages were stimulated with B2M for 6 and 24 h. Following stimulation, cell surface marker (CD80, CD163, CD206), cytokine [interleukin (IL)-6, IL-8, tumor necrosis factor a (TNF-a)] and matrix metalloprotease (MMP; MMP-9 and MMP-13) genes were evaluated by real-time PCR. Additionally, cytokine concentrations in cell culture supernatant were determined using enzyme-linked immunosorbent assay (ELISA). B2M significantly increased CD80 and decreased CD163 expression. In addition, B2M stimulation increased inflammatory cytokines at both the mRNA and protein levels. While B2M likewise elevated MMP-13 levels, there was no difference in MMP-9 expression between vehicle and B2M-treated cells. B2M increased M1 macrophage marker, inflammatory cytokine, and MMP-13 expression in synovial macrophages. B2M-related activation of synovial macrophages may thus be associated with OA pathology. |
| first_indexed | 2024-04-09T16:52:02Z |
| format | Article |
| id | doaj.art-2ada3ce557074a62a2a17b6643ccdd90 |
| institution | Directory Open Access Journal |
| issn | 1426-3912 1644-4124 |
| language | English |
| last_indexed | 2024-04-09T16:52:02Z |
| publishDate | 2023-01-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Central European Journal of Immunology |
| spelling | doaj.art-2ada3ce557074a62a2a17b6643ccdd902023-04-21T09:59:34ZengTermedia Publishing HouseCentral European Journal of Immunology1426-39121644-41242023-01-0147433233810.5114/ceji.2022.12409249898β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synoviumKyoko MuneshigeKentaro UchidaJun AikawaDai IwaseShotaro TakanoMasayuki MiyagiGen InoueMasashi TakasoSeveral studies have implicated b2-microglobulin (B2M) in osteoarthritis (OA) pathology. Of the main constituents of synovial tissue, synovial fibroblasts and macrophages, the latter play a pivotal role in inflammation. Although several studies have investigated the effects of B2M on synovial fibroblasts, few have examined the impact on synovial macrophages. Here, we investigated the effect of B2M on the expression profiles of inflammatory cytokines and matrix metalloproteases (MMPs) in synovial macrophages. Synovial macrophages were isolated from the osteoarthritic synovium using an anti-CD14 anti- body and magnetic isolation system. Synovial macrophages were stimulated with B2M for 6 and 24 h. Following stimulation, cell surface marker (CD80, CD163, CD206), cytokine [interleukin (IL)-6, IL-8, tumor necrosis factor a (TNF-a)] and matrix metalloprotease (MMP; MMP-9 and MMP-13) genes were evaluated by real-time PCR. Additionally, cytokine concentrations in cell culture supernatant were determined using enzyme-linked immunosorbent assay (ELISA). B2M significantly increased CD80 and decreased CD163 expression. In addition, B2M stimulation increased inflammatory cytokines at both the mRNA and protein levels. While B2M likewise elevated MMP-13 levels, there was no difference in MMP-9 expression between vehicle and B2M-treated cells. B2M increased M1 macrophage marker, inflammatory cytokine, and MMP-13 expression in synovial macrophages. B2M-related activation of synovial macrophages may thus be associated with OA pathology.https://www.termedia.pl/-2-microglobulin-alters-the-profiles-of-inflammatory-cytokines-and-of-matrix-metalloprotease-in-macrophages-derived-from-the-osteoarthritic-synovium,10,49898,1,1.htmlosteoarthritis macrophages b2-microglobulin |
| spellingShingle | Kyoko Muneshige Kentaro Uchida Jun Aikawa Dai Iwase Shotaro Takano Masayuki Miyagi Gen Inoue Masashi Takaso β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium Central European Journal of Immunology osteoarthritis macrophages b2-microglobulin |
| title | β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| title_full | β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| title_fullStr | β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| title_full_unstemmed | β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| title_short | β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| title_sort | β2 microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium |
| topic | osteoarthritis macrophages b2-microglobulin |
| url | https://www.termedia.pl/-2-microglobulin-alters-the-profiles-of-inflammatory-cytokines-and-of-matrix-metalloprotease-in-macrophages-derived-from-the-osteoarthritic-synovium,10,49898,1,1.html |
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