Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes
Mastic gum is a resinous sap produced by <i>Pistacia lentiscus</i> growing in the island of Chios (Greece) and has been recognized since Antiquity for its distinctive aroma as well as medical properties (antimicrobial, antioxidant, anti-inflammatory ones). The oral absorption of Chios Ma...
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/3/271 |
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| author | Olga Gortzi Magdalini Rovoli Konstantinos Katsoulis Konstantia Graikou Despoina-Aikaterini Karagkini Dimitrios Stagos Dimitrios Kouretas John Tsaknis Ioanna Chinou |
| author_facet | Olga Gortzi Magdalini Rovoli Konstantinos Katsoulis Konstantia Graikou Despoina-Aikaterini Karagkini Dimitrios Stagos Dimitrios Kouretas John Tsaknis Ioanna Chinou |
| author_sort | Olga Gortzi |
| collection | DOAJ |
| description | Mastic gum is a resinous sap produced by <i>Pistacia lentiscus</i> growing in the island of Chios (Greece) and has been recognized since Antiquity for its distinctive aroma as well as medical properties (antimicrobial, antioxidant, anti-inflammatory ones). The oral absorption of Chios Mastic gum (an insoluble polymer of poly-β-myrcene is among the most abundant contents) is poor due to its low water-solubility. We report in this study, two different Chios mastic gum extracts, the acidic mastic gum extract—AMGE—and the neutral one—NMGE, both prepared after removal of the contained polymer in order to ameliorate solubility and enhance in vivo activity. Liposomes are presented as a promising delivery system due to their physicochemical and biophysical properties to increase stability and absorption efficiency of the mastic gum extracts within the gastrointestinal (GI) tract. The aim of this study was to evaluate the stability in GI simulated conditions together with cytotoxic and antimicrobial activity of the two extracts (AMGE and NMGE) after encapsulation in a well characterized liposome formulation. Liposomes-AMGE complex showed an improved stability behavior in GI simulated conditions. Both assayed extracts showed significant dose dependent inhibition against the growth of liver cancer HepG2 cells and an interesting antimicrobial activity against several microorganisms. Conclusively, encapsulation could be evaluated as a beneficial procedure for further applications of mastic resin. |
| first_indexed | 2024-03-09T23:54:18Z |
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| id | doaj.art-2af1b0a298d44d9aa21f2c7b99b3db03 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:54:18Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2af1b0a298d44d9aa21f2c7b99b3db032023-11-23T16:28:10ZengMDPI AGFoods2304-81582022-01-0111327110.3390/foods11030271Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in LiposomesOlga Gortzi0Magdalini Rovoli1Konstantinos Katsoulis2Konstantia Graikou3Despoina-Aikaterini Karagkini4Dimitrios Stagos5Dimitrios Kouretas6John Tsaknis7Ioanna Chinou8Department of Agriculture Crop Production and Rural Environment, University of Thessaly, 38446 Volos, GreeceLaboratory of Biochemistry, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceLaboratory of Biochemistry, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa, GreeceLaboratory of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis-Zografou, 15771 Athens, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41110 Larissa, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41110 Larissa, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41110 Larissa, GreeceDepartment of Food Science and Technology, School of Food Science, University of West Attica, 12243 Egaleo, GreeceLaboratory of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis-Zografou, 15771 Athens, GreeceMastic gum is a resinous sap produced by <i>Pistacia lentiscus</i> growing in the island of Chios (Greece) and has been recognized since Antiquity for its distinctive aroma as well as medical properties (antimicrobial, antioxidant, anti-inflammatory ones). The oral absorption of Chios Mastic gum (an insoluble polymer of poly-β-myrcene is among the most abundant contents) is poor due to its low water-solubility. We report in this study, two different Chios mastic gum extracts, the acidic mastic gum extract—AMGE—and the neutral one—NMGE, both prepared after removal of the contained polymer in order to ameliorate solubility and enhance in vivo activity. Liposomes are presented as a promising delivery system due to their physicochemical and biophysical properties to increase stability and absorption efficiency of the mastic gum extracts within the gastrointestinal (GI) tract. The aim of this study was to evaluate the stability in GI simulated conditions together with cytotoxic and antimicrobial activity of the two extracts (AMGE and NMGE) after encapsulation in a well characterized liposome formulation. Liposomes-AMGE complex showed an improved stability behavior in GI simulated conditions. Both assayed extracts showed significant dose dependent inhibition against the growth of liver cancer HepG2 cells and an interesting antimicrobial activity against several microorganisms. Conclusively, encapsulation could be evaluated as a beneficial procedure for further applications of mastic resin.https://www.mdpi.com/2304-8158/11/3/271chios mastic gumliposomesencapsulationstabilitycytotoxic activityantimicrobial activity |
| spellingShingle | Olga Gortzi Magdalini Rovoli Konstantinos Katsoulis Konstantia Graikou Despoina-Aikaterini Karagkini Dimitrios Stagos Dimitrios Kouretas John Tsaknis Ioanna Chinou Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes Foods chios mastic gum liposomes encapsulation stability cytotoxic activity antimicrobial activity |
| title | Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes |
| title_full | Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes |
| title_fullStr | Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes |
| title_full_unstemmed | Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes |
| title_short | Study of Stability, Cytotoxic and Antimicrobial Activity of Chios Mastic Gum Fractions (Neutral, Acidic) after Encapsulation in Liposomes |
| title_sort | study of stability cytotoxic and antimicrobial activity of chios mastic gum fractions neutral acidic after encapsulation in liposomes |
| topic | chios mastic gum liposomes encapsulation stability cytotoxic activity antimicrobial activity |
| url | https://www.mdpi.com/2304-8158/11/3/271 |
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