CLAMP and Zelda function together to promote Drosophila zygotic genome activation
During the essential and conserved process of zygotic genome activation (ZGA), chromatin accessibility must increase to promote transcription. Drosophila is a well-established model for defining mechanisms that drive ZGA. Zelda (ZLD) is a key pioneer transcription factor (TF) that promotes ZGA in th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/69937 |
| _version_ | 1811200147633209344 |
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| author | Jingyue Duan Leila Rieder Megan M Colonnetta Annie Huang Mary Mckenney Scott Watters Girish Deshpande William Jordan Nicolas Fawzi Erica Larschan |
| author_facet | Jingyue Duan Leila Rieder Megan M Colonnetta Annie Huang Mary Mckenney Scott Watters Girish Deshpande William Jordan Nicolas Fawzi Erica Larschan |
| author_sort | Jingyue Duan |
| collection | DOAJ |
| description | During the essential and conserved process of zygotic genome activation (ZGA), chromatin accessibility must increase to promote transcription. Drosophila is a well-established model for defining mechanisms that drive ZGA. Zelda (ZLD) is a key pioneer transcription factor (TF) that promotes ZGA in the Drosophila embryo. However, many genomic loci that contain GA-rich motifs become accessible during ZGA independent of ZLD. Therefore, we hypothesized that other early TFs that function with ZLD have not yet been identified, especially those that are capable of binding to GA-rich motifs such as chromatin-linked adaptor for male-specific lethal (MSL) proteins (CLAMP). Here, we demonstrate that Drosophila embryonic development requires maternal CLAMP to (1) activate zygotic transcription; (2) increase chromatin accessibility at promoters of specific genes that often encode other essential TFs; and (3) enhance chromatin accessibility and facilitate ZLD occupancy at a subset of key embryonic promoters. Thus, CLAMP functions as a pioneer factor that plays a targeted yet essential role in ZGA. |
| first_indexed | 2024-04-12T02:00:00Z |
| format | Article |
| id | doaj.art-2af79af3120c48578eb73149fd9fe6f2 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:00:00Z |
| publishDate | 2021-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2af79af3120c48578eb73149fd9fe6f22022-12-22T03:52:42ZengeLife Sciences Publications LtdeLife2050-084X2021-08-011010.7554/eLife.69937CLAMP and Zelda function together to promote Drosophila zygotic genome activationJingyue Duan0https://orcid.org/0000-0001-6416-2250Leila Rieder1Megan M Colonnetta2https://orcid.org/0000-0001-5685-1670Annie Huang3Mary Mckenney4Scott Watters5Girish Deshpande6William Jordan7Nicolas Fawzi8Erica Larschan9https://orcid.org/0000-0003-2484-4921Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United StatesDepartment of Biology, Emory University, Atlanta, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United StatesDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United StatesDepartment of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, United StatesDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United States; Department of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United StatesDepartment of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, United StatesDepartment of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, United StatesDuring the essential and conserved process of zygotic genome activation (ZGA), chromatin accessibility must increase to promote transcription. Drosophila is a well-established model for defining mechanisms that drive ZGA. Zelda (ZLD) is a key pioneer transcription factor (TF) that promotes ZGA in the Drosophila embryo. However, many genomic loci that contain GA-rich motifs become accessible during ZGA independent of ZLD. Therefore, we hypothesized that other early TFs that function with ZLD have not yet been identified, especially those that are capable of binding to GA-rich motifs such as chromatin-linked adaptor for male-specific lethal (MSL) proteins (CLAMP). Here, we demonstrate that Drosophila embryonic development requires maternal CLAMP to (1) activate zygotic transcription; (2) increase chromatin accessibility at promoters of specific genes that often encode other essential TFs; and (3) enhance chromatin accessibility and facilitate ZLD occupancy at a subset of key embryonic promoters. Thus, CLAMP functions as a pioneer factor that plays a targeted yet essential role in ZGA.https://elifesciences.org/articles/69937zygotic genome activationCLAMPZeldaDrosophila embryopioneer TF |
| spellingShingle | Jingyue Duan Leila Rieder Megan M Colonnetta Annie Huang Mary Mckenney Scott Watters Girish Deshpande William Jordan Nicolas Fawzi Erica Larschan CLAMP and Zelda function together to promote Drosophila zygotic genome activation eLife zygotic genome activation CLAMP Zelda Drosophila embryo pioneer TF |
| title | CLAMP and Zelda function together to promote Drosophila zygotic genome activation |
| title_full | CLAMP and Zelda function together to promote Drosophila zygotic genome activation |
| title_fullStr | CLAMP and Zelda function together to promote Drosophila zygotic genome activation |
| title_full_unstemmed | CLAMP and Zelda function together to promote Drosophila zygotic genome activation |
| title_short | CLAMP and Zelda function together to promote Drosophila zygotic genome activation |
| title_sort | clamp and zelda function together to promote drosophila zygotic genome activation |
| topic | zygotic genome activation CLAMP Zelda Drosophila embryo pioneer TF |
| url | https://elifesciences.org/articles/69937 |
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