Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation
ABSTRACTThe spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-04-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00642-23 |
| _version_ | 1827275849863266304 |
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| author | Christopher Gager Ana L. Flores-Mireles |
| author_facet | Christopher Gager Ana L. Flores-Mireles |
| author_sort | Christopher Gager |
| collection | DOAJ |
| description | ABSTRACTThe spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a powerful component of microbial pathogenesis, providing a scaffold for efficient colonization and shielding against anti-microbials, which further complicates drug resistance studies. Early genetic knockout tools didn’t allow the study of essential genes, but clustered regularly interspaced palindromic repeat inference (CRISPRi) technologies have overcome this challenge via genetic silencing. These tools rapidly evolved to meet new demands and exploit native CRISPR systems. Modern tools range from the creation of massive CRISPRi libraries to tunable modulation of gene expression with CRISPR activation (CRISPRa). This review discusses the rapid expansion of CRISPRi/a-based technologies, their use in investigating MDR and biofilm formation, and how this drives further development of a potent tool to comprehensively examine multi-drug resistance. |
| first_indexed | 2024-04-24T05:49:51Z |
| format | Article |
| id | doaj.art-2b43841f2e994c3da2b8b15fc98815cf |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-04-24T05:49:51Z |
| publishDate | 2024-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-2b43841f2e994c3da2b8b15fc98815cf2024-04-23T13:00:39ZengAmerican Society for MicrobiologymSphere2379-50422024-04-019410.1128/msphere.00642-23Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formationChristopher Gager0Ana L. Flores-Mireles1Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USADepartment of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USAABSTRACTThe spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a powerful component of microbial pathogenesis, providing a scaffold for efficient colonization and shielding against anti-microbials, which further complicates drug resistance studies. Early genetic knockout tools didn’t allow the study of essential genes, but clustered regularly interspaced palindromic repeat inference (CRISPRi) technologies have overcome this challenge via genetic silencing. These tools rapidly evolved to meet new demands and exploit native CRISPR systems. Modern tools range from the creation of massive CRISPRi libraries to tunable modulation of gene expression with CRISPR activation (CRISPRa). This review discusses the rapid expansion of CRISPRi/a-based technologies, their use in investigating MDR and biofilm formation, and how this drives further development of a potent tool to comprehensively examine multi-drug resistance.https://journals.asm.org/doi/10.1128/msphere.00642-23antimicrobialCRISPRifungibacteriamulti-drug resistancebiofilms |
| spellingShingle | Christopher Gager Ana L. Flores-Mireles Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation mSphere antimicrobial CRISPRi fungi bacteria multi-drug resistance biofilms |
| title | Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation |
| title_full | Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation |
| title_fullStr | Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation |
| title_full_unstemmed | Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation |
| title_short | Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation |
| title_sort | blunted blades new crispr derived technologies to dissect microbial multi drug resistance and biofilm formation |
| topic | antimicrobial CRISPRi fungi bacteria multi-drug resistance biofilms |
| url | https://journals.asm.org/doi/10.1128/msphere.00642-23 |
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