Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation
The aim of this study was to investigate the effects of bergamot polysaccharide (BP) and Laoxianghuang polysaccharides (LPs, fermented bergamot) on the microbiome and metabolome during the in vitro fermentation of gut microbiota from patients with hyperlipidemia. Results indicated that both BP and L...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/14/2039 |
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| author | Yang Zheng Yi Wang Donghui Luo Lianzhu Lin Xingyu Lu Jie Gao Chuqiao Xiao Mouming Zhao |
| author_facet | Yang Zheng Yi Wang Donghui Luo Lianzhu Lin Xingyu Lu Jie Gao Chuqiao Xiao Mouming Zhao |
| author_sort | Yang Zheng |
| collection | DOAJ |
| description | The aim of this study was to investigate the effects of bergamot polysaccharide (BP) and Laoxianghuang polysaccharides (LPs, fermented bergamot) on the microbiome and metabolome during the in vitro fermentation of gut microbiota from patients with hyperlipidemia. Results indicated that both BP and LPs were able to increase the production of acetic acid, propionic acid, and butyric acid. However, only LPs could decrease the content of isobutyric acid and isovaleric acid, which are detrimental to gut health. A 16S rRNA analysis showed that both BP and LPs could reduce the proportion of <i>Fusobacterium</i>, whereas they increased the <i>Bacteroides</i> content in hyperlipidemia. Untargeted UPLC-MS/MS metabolomic profiling found six bio-markers that were significantly changed after BP and LPs intervention, and four of the down-regulated metabolites were long-chain fatty acids associated with vascular diseases. These findings provide new evidence that BP and LPs have the potential to regulate imbalances in the gut microbiota in patients with hyperlipidemia and ameliorate its metabolic abnormalities. |
| first_indexed | 2024-03-09T10:19:03Z |
| format | Article |
| id | doaj.art-2b4686fb415b4c33bf90d3732a7b5f1c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T10:19:03Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2b4686fb415b4c33bf90d3732a7b5f1c2023-12-01T22:08:51ZengMDPI AGFoods2304-81582022-07-011114203910.3390/foods11142039Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro FermentationYang Zheng0Yi Wang1Donghui Luo2Lianzhu Lin3Xingyu Lu4Jie Gao5Chuqiao Xiao6Mouming Zhao7Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, ChinaJinhua Academy of Agricultural Sciences, Jinhua 321000, ChinaChaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, ChinaChaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, ChinaSchool of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaSchool of Light Industry and Food Engineering, Guangxi University, Nanning 530004, ChinaChaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaThe aim of this study was to investigate the effects of bergamot polysaccharide (BP) and Laoxianghuang polysaccharides (LPs, fermented bergamot) on the microbiome and metabolome during the in vitro fermentation of gut microbiota from patients with hyperlipidemia. Results indicated that both BP and LPs were able to increase the production of acetic acid, propionic acid, and butyric acid. However, only LPs could decrease the content of isobutyric acid and isovaleric acid, which are detrimental to gut health. A 16S rRNA analysis showed that both BP and LPs could reduce the proportion of <i>Fusobacterium</i>, whereas they increased the <i>Bacteroides</i> content in hyperlipidemia. Untargeted UPLC-MS/MS metabolomic profiling found six bio-markers that were significantly changed after BP and LPs intervention, and four of the down-regulated metabolites were long-chain fatty acids associated with vascular diseases. These findings provide new evidence that BP and LPs have the potential to regulate imbalances in the gut microbiota in patients with hyperlipidemia and ameliorate its metabolic abnormalities.https://www.mdpi.com/2304-8158/11/14/2039polysaccharidehyperlipidemicgut microbiotaSCFAsmetabolomics |
| spellingShingle | Yang Zheng Yi Wang Donghui Luo Lianzhu Lin Xingyu Lu Jie Gao Chuqiao Xiao Mouming Zhao Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation Foods polysaccharide hyperlipidemic gut microbiota SCFAs metabolomics |
| title | Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation |
| title_full | Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation |
| title_fullStr | Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation |
| title_full_unstemmed | Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation |
| title_short | Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation |
| title_sort | effect of bergamot and laoxianghuang polysaccharides on gut microbiota derived from patients with hyperlipidemia an integrative analysis of microbiome and metabolome during in vitro fermentation |
| topic | polysaccharide hyperlipidemic gut microbiota SCFAs metabolomics |
| url | https://www.mdpi.com/2304-8158/11/14/2039 |
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