Activity-based protein profiling of a surfactin-producing nonribosomal peptide synthetase in Bacillus subtilis

Activity-based protein profiling of a surfactin-producing nonribosomal peptide synthetase in Bacillus subtilis

Summary: We present an in vitro and in-cell activity-based protein profiling (ABPP) protocol for endogenous nonribosomal peptide synthetases (NRPSs). This protocol enables the fluorescence labeling and imaging of an endogenous SrfAB-NRPS with high selectivity and sensitivity in the surfactin produce...

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Bibliographic Details
Main Authors: Fumihiro Ishikawa, Rina Ohnishi, Chiharu Uchida, Genzoh Tanabe
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Microbiology
Molecular/Chemical Probes
Protein Biochemistry
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003422

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http://www.sciencedirect.com/science/article/pii/S2666166722003422

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