Affinity and Structural Analysis of the U1A RNA Recognition Motif with Engineered Methionines to Improve Experimental Phasing

Affinity and Structural Analysis of the U1A RNA Recognition Motif with Engineered Methionines to Improve Experimental Phasing

RNA plays a central role in all organisms and can fold into complex structures to orchestrate function. Visualization of such structures often requires crystallization, which can be a bottleneck in the structure-determination process. To promote crystallization, an RNA-recognition motif (RRM) of the...

Full description

Bibliographic Details
Main Authors: Yoshita Srivastava, Rachel Bonn-Breach, Sai Shashank Chavali, Geoffrey M. Lippa, Jermaine L. Jenkins, Joseph E. Wedekind
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Crystals
Subjects:
RNA-protein interactions
RNA crystallization
isothermal titration calorimetry
X-ray crystallography
selenomethionine
anomalous diffraction
Online Access:https://www.mdpi.com/2073-4352/11/3/273

Internet

https://www.mdpi.com/2073-4352/11/3/273

Similar Items