Phosphatase specificity principles uncovered by MRBLE:Dephos and global substrate identification

Phosphatase specificity principles uncovered by MRBLE:Dephos and global substrate identification

Abstract Phosphoprotein phosphatases (PPPs) regulate major signaling pathways, but the determinants of phosphatase specificity are poorly understood. This is because methods to investigate this at scale are lacking. Here, we develop a novel in vitro assay, MRBLE:Dephos, that allows multiplexing of d...

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Bibliographic Details
Main Authors: Jamin B Hein, Hieu T Nguyen, Dimitriya H Garvanska, Isha Nasa, Thomas Kruse, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N Kettenbach, Polly M Fordyce, Jakob Nilsson
Format: Article
Language:English
Published: Springer Nature 2023-12-01
Series:Molecular Systems Biology
Subjects:
mitotic exit
MRBLE:Dephos
MRBLE‐Pep
protein phosphatase
substrates
Online Access:https://doi.org/10.15252/msb.202311782

Internet

https://doi.org/10.15252/msb.202311782

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