Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems

Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems

Abstract Cell‐free expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cell‐free measurements is limited by the use of channel‐limited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cell‐Free Transcription and Sequencin...

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Bibliographic Details
Main Authors: Sung Sun Yim, Nathan I Johns, Jimin Park, Antonio LC Gomes, Ross M McBee, Miles Richardson, Carlotta Ronda, Sway P Chen, David Garenne, Vincent Noireaux, Harris H Wang
Format: Article
Language:English
Published: Springer Nature 2019-08-01
Series:Molecular Systems Biology
Subjects:
cell‐free expression systems
gene expression
massively parallel reporter assay
synthetic biology
transcription
Online Access:https://doi.org/10.15252/msb.20198875

Internet

https://doi.org/10.15252/msb.20198875

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