Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells
Glucose-induced oxidative stress is associated with the overproduction of reactive oxygen species (ROS), which may dysregulate the expression of genes controlling insulin secretion leading to β-cell dysfunction, a hallmark of type 2 diabetes mellitus (T2DM). This study investigated the impact of col...
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/9/3314 |
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| author | Gideon Gatluak Kang Nidhish Francis Rodney Hill Daniel LE Waters Christopher L. Blanchard Abishek Bommannan Santhakumar |
| author_facet | Gideon Gatluak Kang Nidhish Francis Rodney Hill Daniel LE Waters Christopher L. Blanchard Abishek Bommannan Santhakumar |
| author_sort | Gideon Gatluak Kang |
| collection | DOAJ |
| description | Glucose-induced oxidative stress is associated with the overproduction of reactive oxygen species (ROS), which may dysregulate the expression of genes controlling insulin secretion leading to β-cell dysfunction, a hallmark of type 2 diabetes mellitus (T2DM). This study investigated the impact of coloured rice phenolic extracts (CRPEs) on the expression of key genes associated with β-cell function in pancreatic β-cells (INS-1E). These genes included glucose transporter 2 (<i>Glut2</i>)<i>,</i> silent mating type information regulation 2 homolog 1 (<i>Sirt1</i>)<i>,</i> mitochondrial transcription factor A (<i>Tfam</i>)<i>,</i> pancreatic/duodenal homeobox protein 1 (<i>Pdx-1</i>) and insulin 1 (<i>Ins1</i>). INS-1E cells were cultured in high glucose (25 mM) to induce glucotoxic stress conditions (HGSC) and in normal glucose conditions (NGC-11.1 mM) to represent normal β-cell function. Cells were treated with CRPEs derived from two coloured rice cultivars, Purple and Yunlu29-red varieties at concentrations ranged from 50 to 250 µg/mL. CRPEs upregulated the expression of <i>Glut2, Sirt1</i> and <i>Pdx-1</i> significantly at 250 µg/mL under HGSC. CRPEs from both cultivars also upregulated <i>Glut2, Sirt1, Tfam, Pdx-1</i> and <i>Ins1</i> markedly at 250 µg/mL under NGC with Yunlu29 having the greatest effect. These data suggest that CRPEs may reduce β-cell dysfunction in T2DM by upregulating the expression of genes involved in insulin secretion pathways. |
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| language | English |
| last_indexed | 2024-03-10T19:59:07Z |
| publishDate | 2020-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2b90f74040604442bf091cde657b0db82023-11-19T23:43:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-01219331410.3390/ijms21093314Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cellsGideon Gatluak Kang0Nidhish Francis1Rodney Hill2Daniel LE Waters3Christopher L. Blanchard4Abishek Bommannan Santhakumar5Australian Research Council (ARC) Industrial Transformation Training Centre (ITTC) for Functional Grains, Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, AustraliaAustralian Research Council (ARC) Industrial Transformation Training Centre (ITTC) for Functional Grains, Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, AustraliaSchool of Biomedical Sciences, Charles Sturt University, NSW 2650, AustraliaAustralian Research Council (ARC) Industrial Transformation Training Centre (ITTC) for Functional Grains, Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, AustraliaAustralian Research Council (ARC) Industrial Transformation Training Centre (ITTC) for Functional Grains, Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, AustraliaAustralian Research Council (ARC) Industrial Transformation Training Centre (ITTC) for Functional Grains, Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, AustraliaGlucose-induced oxidative stress is associated with the overproduction of reactive oxygen species (ROS), which may dysregulate the expression of genes controlling insulin secretion leading to β-cell dysfunction, a hallmark of type 2 diabetes mellitus (T2DM). This study investigated the impact of coloured rice phenolic extracts (CRPEs) on the expression of key genes associated with β-cell function in pancreatic β-cells (INS-1E). These genes included glucose transporter 2 (<i>Glut2</i>)<i>,</i> silent mating type information regulation 2 homolog 1 (<i>Sirt1</i>)<i>,</i> mitochondrial transcription factor A (<i>Tfam</i>)<i>,</i> pancreatic/duodenal homeobox protein 1 (<i>Pdx-1</i>) and insulin 1 (<i>Ins1</i>). INS-1E cells were cultured in high glucose (25 mM) to induce glucotoxic stress conditions (HGSC) and in normal glucose conditions (NGC-11.1 mM) to represent normal β-cell function. Cells were treated with CRPEs derived from two coloured rice cultivars, Purple and Yunlu29-red varieties at concentrations ranged from 50 to 250 µg/mL. CRPEs upregulated the expression of <i>Glut2, Sirt1</i> and <i>Pdx-1</i> significantly at 250 µg/mL under HGSC. CRPEs from both cultivars also upregulated <i>Glut2, Sirt1, Tfam, Pdx-1</i> and <i>Ins1</i> markedly at 250 µg/mL under NGC with Yunlu29 having the greatest effect. These data suggest that CRPEs may reduce β-cell dysfunction in T2DM by upregulating the expression of genes involved in insulin secretion pathways.https://www.mdpi.com/1422-0067/21/9/3314coloured ricepolyphenolsgene expressionpancreatic β-cells functioninsulin secretiontype 2 diabetes |
| spellingShingle | Gideon Gatluak Kang Nidhish Francis Rodney Hill Daniel LE Waters Christopher L. Blanchard Abishek Bommannan Santhakumar Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells International Journal of Molecular Sciences coloured rice polyphenols gene expression pancreatic β-cells function insulin secretion type 2 diabetes |
| title | Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells |
| title_full | Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells |
| title_fullStr | Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells |
| title_full_unstemmed | Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells |
| title_short | Coloured Rice Phenolic Extracts Increase Expression of Genes Associated with Insulin Secretion in Rat Pancreatic Insulinoma β-cells |
| title_sort | coloured rice phenolic extracts increase expression of genes associated with insulin secretion in rat pancreatic insulinoma β cells |
| topic | coloured rice polyphenols gene expression pancreatic β-cells function insulin secretion type 2 diabetes |
| url | https://www.mdpi.com/1422-0067/21/9/3314 |
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