Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays

The influence of time and temperature on the storage of an alkaline antigen of L.major-like and L.(V.) braziliensis promastigotes added or not of a proteases inhibitor (PMSF) was evaluted by means of an IgG-ELISA. Antibodies in assays using L. major-like antigen stored at -20oC for 6 monsths had a s...

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Bibliographic Details
Main Authors: Celeste Beatriz Julieta, Guimarães Maria Carolina Soares, Souza José Maria Pacheco de
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 1997-01-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000400005