Snapshot multicolor fluorescence imaging using double multiplexing of excitation and emission on a single detector

Abstract Fluorescence-based multispectral imaging of rapidly moving or dynamic samples requires both fast two-dimensional data acquisition as well as sufficient spectral sensitivity for species separation. As the number of fluorophores in the experiment increases, meeting both these requirements bec...

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Bibliographic Details
Main Authors: Karolina Dorozynska, Simon Ek, Vassily Kornienko, David Andersson, Alexandra Andersson, Andreas Ehn, Elias Kristensson
Format: Article
Language:English
Published: Nature Portfolio 2021-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-99670-6