Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs
Mycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased inMycoplasma hyorhinis‐infected tumor cell cultures compared with non‐infected tumor cell cultures. This mycoplasma‐driven drug deamination could be prevented by exogenous administrat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.fob.2015.07.007 |
| _version_ | 1811185139617628160 |
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| author | Johan Vande Voorde Peter Vervaeke Sandra Liekens Jan Balzarini |
| author_facet | Johan Vande Voorde Peter Vervaeke Sandra Liekens Jan Balzarini |
| author_sort | Johan Vande Voorde |
| collection | DOAJ |
| description | Mycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased inMycoplasma hyorhinis‐infected tumor cell cultures compared with non‐infected tumor cell cultures. This mycoplasma‐driven drug deamination could be prevented by exogenous administration of the cytidine deaminase (CDA) inhibitor tetrahydrouridine, but also by the natural nucleosides or by a purine nucleoside phosphorylase inhibitor. TheM. hyorhinis‐encoded CDAHyor gene was cloned, expressed as a recombinant protein and purified. CDAHyor was found to be more catalytically active than its human equivalent and efficiently deaminates (inactivates) cytosine‐based anticancer drugs. CDAHyor expression at the tumor site may result in selective drug inactivation and suboptimal therapeutic efficiency. |
| first_indexed | 2024-04-11T13:24:21Z |
| format | Article |
| id | doaj.art-2bc2a4194b7c43e3b61abfc08e5ec7e8 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T13:24:21Z |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-2bc2a4194b7c43e3b61abfc08e5ec7e82022-12-22T04:22:07ZengWileyFEBS Open Bio2211-54632015-01-015163463910.1016/j.fob.2015.07.007Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugsJohan Vande Voorde0Peter Vervaeke1Sandra Liekens2Jan Balzarini3Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, blok x – bus 1030, B-3000 Leuven, BelgiumRega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, blok x – bus 1030, B-3000 Leuven, BelgiumRega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, blok x – bus 1030, B-3000 Leuven, BelgiumRega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, blok x – bus 1030, B-3000 Leuven, BelgiumMycoplasmas may colonize tumor tissue in patients. The cytostatic activity of gemcitabine was dramatically decreased inMycoplasma hyorhinis‐infected tumor cell cultures compared with non‐infected tumor cell cultures. This mycoplasma‐driven drug deamination could be prevented by exogenous administration of the cytidine deaminase (CDA) inhibitor tetrahydrouridine, but also by the natural nucleosides or by a purine nucleoside phosphorylase inhibitor. TheM. hyorhinis‐encoded CDAHyor gene was cloned, expressed as a recombinant protein and purified. CDAHyor was found to be more catalytically active than its human equivalent and efficiently deaminates (inactivates) cytosine‐based anticancer drugs. CDAHyor expression at the tumor site may result in selective drug inactivation and suboptimal therapeutic efficiency.https://doi.org/10.1016/j.fob.2015.07.007MycoplasmaCytidine deaminaseCancerNucleoside analoguePurine nucleoside phosphorylaseGemcitabine |
| spellingShingle | Johan Vande Voorde Peter Vervaeke Sandra Liekens Jan Balzarini Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs FEBS Open Bio Mycoplasma Cytidine deaminase Cancer Nucleoside analogue Purine nucleoside phosphorylase Gemcitabine |
| title | Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs |
| title_full | Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs |
| title_fullStr | Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs |
| title_full_unstemmed | Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs |
| title_short | Mycoplasma hyorhinis‐encoded cytidine deaminase efficiently inactivates cytosine‐based anticancer drugs |
| title_sort | mycoplasma hyorhinis encoded cytidine deaminase efficiently inactivates cytosine based anticancer drugs |
| topic | Mycoplasma Cytidine deaminase Cancer Nucleoside analogue Purine nucleoside phosphorylase Gemcitabine |
| url | https://doi.org/10.1016/j.fob.2015.07.007 |
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