The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new specific primers

Abstract Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA -specific pri...

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Bibliographic Details
Main Authors: Shaymaa Abdelmalek, Karim Shokry, Wafy Hamed, Mohammed Abdelnaser, Ashraf Aboubakr, Sameh Abou Elenin, Mohamed Ali, Mohamed Mostafa, Mahmoud Abou-Okada
Format: Article
Language:English
Published: Nature Portfolio 2022-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-14600-4