Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies
Portulacaria afra Jacq (Portulacaceae) is a medicinal plant used traditionally in the treatment of pain and inflammation. This study aimed to evaluate the phytochemical composition, toxicity, antinociceptive, and enzyme inhibition potential of P. afra. The methanol extract (PAME) was prepared throug...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223002460 |
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| author | Sobia Tabassum Saeed Ahmad Kashif ur Rehman Khan Baber Ali Faisal Usman Qaiser Jabeen Muhammad Sajid-ur-Rehman Maqsood Ahmed Hafiz Muhammad Zubair Luay Alkazmi Gaber El-Saber Batiha Qamar-uz- Zaman Abdul Basit |
| author_facet | Sobia Tabassum Saeed Ahmad Kashif ur Rehman Khan Baber Ali Faisal Usman Qaiser Jabeen Muhammad Sajid-ur-Rehman Maqsood Ahmed Hafiz Muhammad Zubair Luay Alkazmi Gaber El-Saber Batiha Qamar-uz- Zaman Abdul Basit |
| author_sort | Sobia Tabassum |
| collection | DOAJ |
| description | Portulacaria afra Jacq (Portulacaceae) is a medicinal plant used traditionally in the treatment of pain and inflammation. This study aimed to evaluate the phytochemical composition, toxicity, antinociceptive, and enzyme inhibition potential of P. afra. The methanol extract (PAME) was prepared through maceration and fractionated with ethanol to ethanol fraction of P. afra (ETPA). Both PAME and ETPA were found to be rich in total phenolic (TPC) and total flavonoid contents (TFC). Similarly, PAME showed the highest antioxidant potential through ABTS 93.16 ± 0.05 mg TE /g of dry extract (D.E.) while ETPA showed maximum results (462 ± 1.44 mg TE/g) in the CUPRAC method. The RP-UHPLC-MS analysis of PAME showed tentative identification of 101 compounds in the positive mode and 14 compounds in the negative mode of ionization while GC–MS profiling gave a total of 15 compounds. The acute oral toxicity study of PAME revealed the safety and biocompatibility of the extract up to a dose of 5000 mg/kg orally in rats. In-vitro data revealed that varying concentrations of P. afra significantly (p < 0.05) inhibited heat-induced BSA denaturation compared to indomethacin in a concentration-dependent manner. PAME suppressed carrageenan-induced paw edema at the 4th hr with maximum inhibition. The analgesic efficacy of PAME was determined by the hotplate, writhing method, and tail-flicking rat models. In the hot plate method, PAME treated groups showed a significant effect (p < 0.0001). While in the writhing model, the PAME treated groups showed a significant (p < 0.0001) anti-nociceptive effect at 200 mg/kg and 400 mg/kg compared to the control group. Similarly, PAME treated groups in the tail flicking model showed a significant (p < 0.001) anti-nociceptive effect at 200 mg/kg and 400 mg/kg. A dose-dependent increase in latency time (8.78 ± 0.090 at (30 min), 11.39 ± 0.005 at (60 min), 12.14 ± 0.01 at (90 min) 15.19 ± 0.03 at (120 min), p < 0.0001 was observed, compared to the control group. Similarly, the extract and fraction showed significant inhibitory potential against tyrosinase in in vitro and in-silico studies. Conclusively the current study unveiled P. afra as a novel non-toxic source with good total antioxidant-mediated anti-inflammatory and analgesic potential. |
| first_indexed | 2024-04-09T17:54:24Z |
| format | Article |
| id | doaj.art-2bd59e0281d74e14bbb1c9ffc46e4c3f |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-09T17:54:24Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-2bd59e0281d74e14bbb1c9ffc46e4c3f2023-04-15T05:52:21ZengElsevierArabian Journal of Chemistry1878-53522023-06-01166104784Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studiesSobia Tabassum0Saeed Ahmad1Kashif ur Rehman Khan2Baber Ali3Faisal Usman4Qaiser Jabeen5Muhammad Sajid-ur-Rehman6Maqsood Ahmed7Hafiz Muhammad Zubair8Luay Alkazmi9Gaber El-Saber Batiha10Qamar-uz- Zaman11Abdul Basit12Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan; Corresponding authors.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan; Corresponding authors.Department of Plant Sciences, Quaid-i-Azam University, Islamabad 15320, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, PakistanDepartment of Pharmacology, Faculty of Pharmacy, The Islamia Univesity of Bahawalpur, PakistanDepartment of Pharmacognosy, Faculty of Pharmacy, The Islamia University of Bahawalpur, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, PakistanDepartment of Pharmacology, Faculty of Pharmacy, The Islamia Univesity of Bahawalpur, PakistanBiology Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, EgyptDepartment of Environmental Sciences, The University of Lahore, Lahore 54590, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand; Drug Delivery System Research Excellent Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandPortulacaria afra Jacq (Portulacaceae) is a medicinal plant used traditionally in the treatment of pain and inflammation. This study aimed to evaluate the phytochemical composition, toxicity, antinociceptive, and enzyme inhibition potential of P. afra. The methanol extract (PAME) was prepared through maceration and fractionated with ethanol to ethanol fraction of P. afra (ETPA). Both PAME and ETPA were found to be rich in total phenolic (TPC) and total flavonoid contents (TFC). Similarly, PAME showed the highest antioxidant potential through ABTS 93.16 ± 0.05 mg TE /g of dry extract (D.E.) while ETPA showed maximum results (462 ± 1.44 mg TE/g) in the CUPRAC method. The RP-UHPLC-MS analysis of PAME showed tentative identification of 101 compounds in the positive mode and 14 compounds in the negative mode of ionization while GC–MS profiling gave a total of 15 compounds. The acute oral toxicity study of PAME revealed the safety and biocompatibility of the extract up to a dose of 5000 mg/kg orally in rats. In-vitro data revealed that varying concentrations of P. afra significantly (p < 0.05) inhibited heat-induced BSA denaturation compared to indomethacin in a concentration-dependent manner. PAME suppressed carrageenan-induced paw edema at the 4th hr with maximum inhibition. The analgesic efficacy of PAME was determined by the hotplate, writhing method, and tail-flicking rat models. In the hot plate method, PAME treated groups showed a significant effect (p < 0.0001). While in the writhing model, the PAME treated groups showed a significant (p < 0.0001) anti-nociceptive effect at 200 mg/kg and 400 mg/kg compared to the control group. Similarly, PAME treated groups in the tail flicking model showed a significant (p < 0.001) anti-nociceptive effect at 200 mg/kg and 400 mg/kg. A dose-dependent increase in latency time (8.78 ± 0.090 at (30 min), 11.39 ± 0.005 at (60 min), 12.14 ± 0.01 at (90 min) 15.19 ± 0.03 at (120 min), p < 0.0001 was observed, compared to the control group. Similarly, the extract and fraction showed significant inhibitory potential against tyrosinase in in vitro and in-silico studies. Conclusively the current study unveiled P. afra as a novel non-toxic source with good total antioxidant-mediated anti-inflammatory and analgesic potential.http://www.sciencedirect.com/science/article/pii/S1878535223002460Portulacaria afraRP-UHPLC-MSAcute oral toxicityAnti-nociceptiveAnti-inflammatoryMolecular docking |
| spellingShingle | Sobia Tabassum Saeed Ahmad Kashif ur Rehman Khan Baber Ali Faisal Usman Qaiser Jabeen Muhammad Sajid-ur-Rehman Maqsood Ahmed Hafiz Muhammad Zubair Luay Alkazmi Gaber El-Saber Batiha Qamar-uz- Zaman Abdul Basit Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies Arabian Journal of Chemistry Portulacaria afra RP-UHPLC-MS Acute oral toxicity Anti-nociceptive Anti-inflammatory Molecular docking |
| title | Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies |
| title_full | Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies |
| title_fullStr | Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies |
| title_full_unstemmed | Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies |
| title_short | Chemical profiling and evaluation of toxicological, antioxidant, anti-inflammatory, anti-nociceptive and tyrosinase inhibitory potential of Portulacaria afra using in-vitro, in-vivo and in-silico studies |
| title_sort | chemical profiling and evaluation of toxicological antioxidant anti inflammatory anti nociceptive and tyrosinase inhibitory potential of portulacaria afra using in vitro in vivo and in silico studies |
| topic | Portulacaria afra RP-UHPLC-MS Acute oral toxicity Anti-nociceptive Anti-inflammatory Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223002460 |
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