Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis

Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis

We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses...

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Bibliographic Details
Main Authors: Yuting Lu, Ruili Zhang, Zhenying Wang, Shuhua Zhou, Yali Song, Lamei Chen, Nan Chen, Wenmin Liu, Canan Ji, Wangli Wu, Li Zhang
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2018-07-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Clouston syndrome
GJB6 gene
Connexin 30
Caspase
Apoptosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900611&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900611&lng=en&tlng=en

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