Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein

Rapid yeast-based screen for Functionally Relevant Amino Acids (RS-FRAA) in a protein

Summary: Here, we describe a fast and cost-effective procedure to generate a large array of mutant proteins and immediately screen for those with altered protein function. This protocol is a modification from three existing approaches: fusion PCR, Saccharomyces cerevisiae in-yeast recombination, and...

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Bibliographic Details
Main Authors: Aditi A. Ghuge, Reuben A. Anderson, Susanne Gottfried, Clément Daube, Siaosi M.B.M.J. Koloamatangi, Anja H. Schiemann, Evelyn Sattlegger
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Model Organisms
Molecular Biology
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004257

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http://www.sciencedirect.com/science/article/pii/S2666166722004257

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