Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation

This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by in...

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Bibliographic Details
Main Author: Aya Ito-Ishida
Format: Article
Language:English
Published: Bio-protocol LLC 2013-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=778&type=0