Generation of 3D ex vivo mouse- and patient-derived glioma explant slice model for integration of confocal time-lapse imaging and spatial analysis

Generation of 3D ex vivo mouse- and patient-derived glioma explant slice model for integration of confocal time-lapse imaging and spatial analysis

Summary: Development of spatial-integrative pre-clinical models is needed for glioblastoma, which are heterogenous tumors with poor prognosis. Here, we present an optimized protocol to generate three-dimensional ex vivo explant slice glioma model from orthotopic tumors, genetically engineered mouse...

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Bibliographic Details
Main Authors: Andrea Comba, Maria Luisa Varela, Syed M. Faisal, Clifford C. Abel, II, Anna E. Argento, Wajd N. Al-Holou, Todd C. Hollon, Jacqueline D. Perelman, Patrick J. Dunn, Sebastien Motsch, Maria G. Castro, Pedro R. Lowenstein
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Cancer
Health Sciences
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001326

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http://www.sciencedirect.com/science/article/pii/S2666166723001326

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