Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese
The Present study was undertaken to assess the prevalence of B. cereus in milk and some milk Products and its survival in yoghurt and Damietta cheese. A total of 100 samples of raw milk, Pasteurized milk, Damietta cheese and yoghurt (25 for each) were collected from supermarkets in Assiut city and...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2023-05-01
|
| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1232 |
| _version_ | 1797657034714251264 |
|---|---|
| author | Salwa S. Thabet Lamiaa. M. Talaat Al Shrief |
| author_facet | Salwa S. Thabet Lamiaa. M. Talaat Al Shrief |
| author_sort | Salwa S. Thabet |
| collection | DOAJ |
| description |
The Present study was undertaken to assess the prevalence of B. cereus in milk and some milk Products and its survival in yoghurt and Damietta cheese. A total of 100 samples of raw milk, Pasteurized milk, Damietta cheese and yoghurt (25 for each) were collected from supermarkets in Assiut city and were examined for isolation of B. cereus. It was isolated from 24, 32, 68 and 20% of the tested samples, respectively. All the isolated strains were confirmed positive for B. cereus except one strain for each raw milk and Damietta cheese. 14.7%, 85.3, 50.0 and 76.5% of the confirmed strains were carried Ces, Nhe, Hbl and cytK genes. Concerning its survival in yoghurt, there was a significant difference between the effect of yoghurt and acidophilus yoghurt on B. cereus growth, where the organism remained viable to the 9th day in yoghurt and failed to be isolated at the 5th day in yoghurt with probiotics. In case of Damietta cheese, 2 and 5% salt were more favorable for the pathogen growth, while 10% salt was inconvenient for its growth. In addition, the inhibitory effect of thyme essential oil was more obvious than the effect of rosemary EO, where the bacterial population was decreased to a count of 2.05 and 2.46 log cfu/ g. at the 2nd week in case of addition of 1.65% thyme EO and could not be detected at the 4th week, whereas the organism could survive to the 6th week in case of rosemary. In conclusion, strict hygienic measures must be implemented during the manufacture of dairy products and addition of biopreservatives to control B. cereus growth is substantial.
|
| first_indexed | 2024-03-11T17:38:33Z |
| format | Article |
| id | doaj.art-2c467ee7e9d74e89bb31ab4b6872fa18 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:33Z |
| publishDate | 2023-05-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-2c467ee7e9d74e89bb31ab4b6872fa182023-10-18T14:21:46ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-05-01133Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta CheeseSalwa S. Thabet0Lamiaa. M. Talaat Al Shrief1Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center (ARC), Egypt. Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center (ARC), Egypt. The Present study was undertaken to assess the prevalence of B. cereus in milk and some milk Products and its survival in yoghurt and Damietta cheese. A total of 100 samples of raw milk, Pasteurized milk, Damietta cheese and yoghurt (25 for each) were collected from supermarkets in Assiut city and were examined for isolation of B. cereus. It was isolated from 24, 32, 68 and 20% of the tested samples, respectively. All the isolated strains were confirmed positive for B. cereus except one strain for each raw milk and Damietta cheese. 14.7%, 85.3, 50.0 and 76.5% of the confirmed strains were carried Ces, Nhe, Hbl and cytK genes. Concerning its survival in yoghurt, there was a significant difference between the effect of yoghurt and acidophilus yoghurt on B. cereus growth, where the organism remained viable to the 9th day in yoghurt and failed to be isolated at the 5th day in yoghurt with probiotics. In case of Damietta cheese, 2 and 5% salt were more favorable for the pathogen growth, while 10% salt was inconvenient for its growth. In addition, the inhibitory effect of thyme essential oil was more obvious than the effect of rosemary EO, where the bacterial population was decreased to a count of 2.05 and 2.46 log cfu/ g. at the 2nd week in case of addition of 1.65% thyme EO and could not be detected at the 4th week, whereas the organism could survive to the 6th week in case of rosemary. In conclusion, strict hygienic measures must be implemented during the manufacture of dairy products and addition of biopreservatives to control B. cereus growth is substantial. https://advetresearch.com/index.php/AVR/article/view/1232 B. cereusmilk yieldProteolyticLipolyticVirulent genes |
| spellingShingle | Salwa S. Thabet Lamiaa. M. Talaat Al Shrief Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese Journal of Advanced Veterinary Research B. cereus milk yield Proteolytic Lipolytic Virulent genes |
| title | Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese |
| title_full | Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese |
| title_fullStr | Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese |
| title_full_unstemmed | Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese |
| title_short | Behavioral Patterns of the Isolated Bacillus cereus Strains from Milk and Some Milk Products in Yoghurt and Damietta Cheese |
| title_sort | behavioral patterns of the isolated bacillus cereus strains from milk and some milk products in yoghurt and damietta cheese |
| topic | B. cereus milk yield Proteolytic Lipolytic Virulent genes |
| url | https://advetresearch.com/index.php/AVR/article/view/1232 |
| work_keys_str_mv | AT salwasthabet behavioralpatternsoftheisolatedbacilluscereusstrainsfrommilkandsomemilkproductsinyoghurtanddamiettacheese AT lamiaamtalaatalshrief behavioralpatternsoftheisolatedbacilluscereusstrainsfrommilkandsomemilkproductsinyoghurtanddamiettacheese |