Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway
Summary: Osteocytes are the mechano-sensors of bones. Large gradient high-static magnetic fields (LG-HMFs) produce stable, high-precision, and non-attenuation mechanical forces. We discovered that magnetic forces opposite to gravity inhibited MLO-Y4 osteocyte proliferation and viability by inducing...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223014426 |
| _version_ | 1797770723935125504 |
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| author | Bin Zhang Xianglin Li Xiaojie Zhou ChenGe Lou Shenghang Wang Huanhuan Lv Gejing Zhang Yanwen Fang Dachuan Yin Peng Shang |
| author_facet | Bin Zhang Xianglin Li Xiaojie Zhou ChenGe Lou Shenghang Wang Huanhuan Lv Gejing Zhang Yanwen Fang Dachuan Yin Peng Shang |
| author_sort | Bin Zhang |
| collection | DOAJ |
| description | Summary: Osteocytes are the mechano-sensors of bones. Large gradient high-static magnetic fields (LG-HMFs) produce stable, high-precision, and non-attenuation mechanical forces. We discovered that magnetic forces opposite to gravity inhibited MLO-Y4 osteocyte proliferation and viability by inducing structural damage and apoptosis. In contrast, magnetic force loading in the same direction as that of gravity promoted the proliferation and inhibited apoptosis of MLO-Y4 osteocytes. Differentially expressed gene (DEG) analysis after magnetic force stimulation indicated that the ECM-integrin-CSK axis responded most significantly to mechanical signals. Wisp2 was the most significant DEG between the 12 T upward and downward groups, showing the highest correlation with the Wnt pathway according to the STRING protein interaction database. Explaining the cellular and molecular mechanisms by which mechanical stimuli influence bone remodeling is currently the focus of osteocyte-related research. Our findings provide insights into the effects of LG-HMFs on bone cells, which have further implications in clinical practice. |
| first_indexed | 2024-03-12T21:27:21Z |
| format | Article |
| id | doaj.art-2c654d3b954346e7bfde775b7d9903c6 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T21:27:21Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2c654d3b954346e7bfde775b7d9903c62023-07-28T04:26:32ZengElsevieriScience2589-00422023-08-01268107365Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathwayBin Zhang0Xianglin Li1Xiaojie Zhou2ChenGe Lou3Shenghang Wang4Huanhuan Lv5Gejing Zhang6Yanwen Fang7Dachuan Yin8Peng Shang9Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China; School of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaResearch & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China; School of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaSchool of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, ChinaResearch & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China; School of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaSchool of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Department of Spine Surgery, Affiliated Longhua People’s Hospital, Southern Medical University, Shenzhen 518057, ChinaSchool of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaResearch & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China; School of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaHeye Health Technology Co., Ltd, Huzhou 313300, ChinaSchool of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, ChinaResearch & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi’an 710072, China; Corresponding authorSummary: Osteocytes are the mechano-sensors of bones. Large gradient high-static magnetic fields (LG-HMFs) produce stable, high-precision, and non-attenuation mechanical forces. We discovered that magnetic forces opposite to gravity inhibited MLO-Y4 osteocyte proliferation and viability by inducing structural damage and apoptosis. In contrast, magnetic force loading in the same direction as that of gravity promoted the proliferation and inhibited apoptosis of MLO-Y4 osteocytes. Differentially expressed gene (DEG) analysis after magnetic force stimulation indicated that the ECM-integrin-CSK axis responded most significantly to mechanical signals. Wisp2 was the most significant DEG between the 12 T upward and downward groups, showing the highest correlation with the Wnt pathway according to the STRING protein interaction database. Explaining the cellular and molecular mechanisms by which mechanical stimuli influence bone remodeling is currently the focus of osteocyte-related research. Our findings provide insights into the effects of LG-HMFs on bone cells, which have further implications in clinical practice.http://www.sciencedirect.com/science/article/pii/S2589004223014426Cellular physiologyMolecular biologyDevelopmental biology |
| spellingShingle | Bin Zhang Xianglin Li Xiaojie Zhou ChenGe Lou Shenghang Wang Huanhuan Lv Gejing Zhang Yanwen Fang Dachuan Yin Peng Shang Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway iScience Cellular physiology Molecular biology Developmental biology |
| title | Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway |
| title_full | Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway |
| title_fullStr | Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway |
| title_full_unstemmed | Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway |
| title_short | Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway |
| title_sort | magneto mechanical stimulation modulates osteocyte fate via the ecm integrin csk axis and wnt pathway |
| topic | Cellular physiology Molecular biology Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223014426 |
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