Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2
Abstract Intestinal stem cells (ISCs) are responsible for intestinal tissue homeostasis and are important for the regeneration of the damaged intestinal epithelia. Through the establishment of ionizing radiation (IR) induced intestinal injury model, we found that a TLR2 agonist, Zymosan-A, promoted...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2022-10-01
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| Series: | Cell Death and Disease |
| Online Access: | https://doi.org/10.1038/s41419-022-05301-x |
| _version_ | 1828141023488901120 |
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| author | Jicong Du Lan Fang Jianpeng Zhao Yike Yu Zhenlan Feng Yuedong Wang Ying Cheng Bailong Li Fu Gao Cong Liu |
| author_facet | Jicong Du Lan Fang Jianpeng Zhao Yike Yu Zhenlan Feng Yuedong Wang Ying Cheng Bailong Li Fu Gao Cong Liu |
| author_sort | Jicong Du |
| collection | DOAJ |
| description | Abstract Intestinal stem cells (ISCs) are responsible for intestinal tissue homeostasis and are important for the regeneration of the damaged intestinal epithelia. Through the establishment of ionizing radiation (IR) induced intestinal injury model, we found that a TLR2 agonist, Zymosan-A, promoted the regeneration of ISCs in vivo and in vitro. Zymosan-A improved the survival of abdominal irradiated mice (81.82% of mice in the treated group vs. 30% of mice in the PBS group), inhibited the radiation damage of intestinal tissue, increased the survival rate of intestinal crypts and the number of ISCs after lethal IR in vivo. Through organoid experiments, we found that Zymosan-A promoted the proliferation and differentiation of ISCs after IR. Remarkably, the results of RNA sequencing and Western Blot (WB) showed that Zymosan-A reduced IR-induced intestinal injury via TLR2 signaling pathway and Wnt signaling pathway and Zymosan-A had no radioprotection on TLR2 KO mice, suggesting that Zymosan-A may play a radioprotective role by targeting TLR2. Moreover, our results revealed that Zymosan-A increased ASCL2, a transcription factor of ISCs, playing a core role in the process of Zymosan-A against IR-induced intestinal injury and likely contributing to the survival of intestinal organoids post-radiation. In conclusion, we demonstrated that Zymosan-A promotes the regeneration of ISCs by upregulating ASCL2. |
| first_indexed | 2024-04-11T19:18:27Z |
| format | Article |
| id | doaj.art-2c6f489c5c774627840baadca7c6eac0 |
| institution | Directory Open Access Journal |
| issn | 2041-4889 |
| language | English |
| last_indexed | 2024-04-11T19:18:27Z |
| publishDate | 2022-10-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death and Disease |
| spelling | doaj.art-2c6f489c5c774627840baadca7c6eac02022-12-22T04:07:22ZengNature Publishing GroupCell Death and Disease2041-48892022-10-01131011110.1038/s41419-022-05301-xZymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2Jicong Du0Lan Fang1Jianpeng Zhao2Yike Yu3Zhenlan Feng4Yuedong Wang5Ying Cheng6Bailong Li7Fu Gao8Cong Liu9Department of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityDepartment of Radiation Medicine, Faculty of Naval Medicine, Naval Medical UniversityAbstract Intestinal stem cells (ISCs) are responsible for intestinal tissue homeostasis and are important for the regeneration of the damaged intestinal epithelia. Through the establishment of ionizing radiation (IR) induced intestinal injury model, we found that a TLR2 agonist, Zymosan-A, promoted the regeneration of ISCs in vivo and in vitro. Zymosan-A improved the survival of abdominal irradiated mice (81.82% of mice in the treated group vs. 30% of mice in the PBS group), inhibited the radiation damage of intestinal tissue, increased the survival rate of intestinal crypts and the number of ISCs after lethal IR in vivo. Through organoid experiments, we found that Zymosan-A promoted the proliferation and differentiation of ISCs after IR. Remarkably, the results of RNA sequencing and Western Blot (WB) showed that Zymosan-A reduced IR-induced intestinal injury via TLR2 signaling pathway and Wnt signaling pathway and Zymosan-A had no radioprotection on TLR2 KO mice, suggesting that Zymosan-A may play a radioprotective role by targeting TLR2. Moreover, our results revealed that Zymosan-A increased ASCL2, a transcription factor of ISCs, playing a core role in the process of Zymosan-A against IR-induced intestinal injury and likely contributing to the survival of intestinal organoids post-radiation. In conclusion, we demonstrated that Zymosan-A promotes the regeneration of ISCs by upregulating ASCL2.https://doi.org/10.1038/s41419-022-05301-x |
| spellingShingle | Jicong Du Lan Fang Jianpeng Zhao Yike Yu Zhenlan Feng Yuedong Wang Ying Cheng Bailong Li Fu Gao Cong Liu Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 Cell Death and Disease |
| title | Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 |
| title_full | Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 |
| title_fullStr | Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 |
| title_full_unstemmed | Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 |
| title_short | Zymosan-A promotes the regeneration of intestinal stem cells by upregulating ASCL2 |
| title_sort | zymosan a promotes the regeneration of intestinal stem cells by upregulating ascl2 |
| url | https://doi.org/10.1038/s41419-022-05301-x |
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