New Method for 5′−Nucleotidase Preparation and Evaluation of Its Catalytic Activity

In this study, we established a new methodology for preparing 5′−nucleotidase (5′−NT) with the aim of enhancing our understanding of its enzyme activity and laying a basis for regulating the content of umami−enhancing nucleotides in pork. 5′−NT was prepared with Sephadex gel filtration and reverse−p...

Full description

Bibliographic Details
Main Authors: Yin Zhang, Qing Zeng, Yingjie Zhang, Pengcheng Zhang, Qing Li, Jiao Zhou, Li Dong, Zhongli Pan
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/13/5/708