New Method for 5′−Nucleotidase Preparation and Evaluation of Its Catalytic Activity
In this study, we established a new methodology for preparing 5′−nucleotidase (5′−NT) with the aim of enhancing our understanding of its enzyme activity and laying a basis for regulating the content of umami−enhancing nucleotides in pork. 5′−NT was prepared with Sephadex gel filtration and reverse−p...
Main Authors: | Yin Zhang, Qing Zeng, Yingjie Zhang, Pengcheng Zhang, Qing Li, Jiao Zhou, Li Dong, Zhongli Pan |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2024-02-01
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Series: | Foods |
Subjects: | |
Online Access: | https://www.mdpi.com/2304-8158/13/5/708 |
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