Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library.

Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library.

Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating...

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Bibliographic Details
Main Authors: Yanan Sun, Baoan Ning, Ming Liu, Xianjun Gao, Xianjun Fan, Jianqing Liu, Zhixian Gao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3302827?pdf=render

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http://europepmc.org/articles/PMC3302827?pdf=render

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